I am working on animal model of motor disorders. I have to estimate the various monoamines (5-HT, NE, DA) in striatum, hippocampus, cortex region of brain. I wish to work on HPLC-electrochemical detection method for that. But problem is that I donot have organic solvents now. It will take some time to come.
My question is
Can I preserve the brain sample in HEPES buffer/PBS/TBS and store in -80degree celcius deep freezer for 1-1.5 months?? Though I will not mince the islotated region of brain parts. That I will do when the solvents for HPLC will be available.
Does this process of cold storage may impact or alter the neurotransmitters level in brain??
What can be the other reliable and sensitive/precise method other than HPLC that works for neurotransmitters, ???
Please the concerned field of the researchers share your opinions, suggestions and views to clear it out....
Thanking you
I think your choice of solvent is wrong. Buffers may block the column. My suggestion is to use either Water (contain 0.1% formic acid) or methanol (contain 0.1% formic acid). You can keep for longer time. -20 is also fine.
We snap freeze the brain tissue samples in 1.5ml eppendorf tubes without any solvent in liquid nitrogen and then we store it in a -80 degrees freezer for up to a 6 months before we do the sample analysis.
We do the same as François. But he had tested 1 year-old samples and they were just as good as fresh samples. So you can store your samples without any solvent.
Completely agree with Francios and we tried this in our previous experiment and used it after 2 months and it was fine
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