Mert Burak Ozturk

9 Questions 12 Answers 0 Followers

Questions related from Mert Burak Ozturk

Extracellular and intracellular Melanin synthesis in B16F10?

Hi all, I have been trying let B16F10 cells to produce and secrete Melanin upon a-MSH induction. Cells were induced with 50nM, 100nM and 500nM a-MSH in complete DMEM including 0.5mM L-tyrosine...

06 June 2023 2,904 0 View

Any resource showing circRNA (circular RNA) sponging (trap) mRNA?

Hi all, I am looking for some resources showing sponging/trapping mRNA? Most of the papers about sponge function of circRNA show only miRNAs or proteins (RBB)/peptides etc. I need specifically...

06 March 2023 9,984 1 View

Is there a way to track origin of the natural product derivatives?

Hi all, I wonder whether there is a way to find the original natural product from the modified structure? It is very common to use natural product derivatives in HTS/HCS/in silicon screening to...

01 March 2023 8,195 3 View

Does the poly(A) tail stop the T7-dependent in vitro transcription?

Dear all, I am planning to do in vitro transcription from a PCR template. However, I need a poly(A) tailed RNA product. If I add 30 nucleotide poly(A) tail before the in vitro transcription,...

23 May 2022 148 3 View

Coating 384-well plate with poly-L-lysine?

Dear all, May I know your 384-well plate poly-L-lysine coating protocol for HEK293 cells? Besides, How sensitive is poly-l-lysine to environment conditions such as leaving the stock at room...

29 August 2021 1,289 2 View

Failure in LR Gateway cloning?

Hi all, Currently, I am having difficulty with Gateway LR cloning. Actually with only one gene. For other genes, the technology works almost %100. However, when I tried to transfer HDAC2 into my...

15 July 2021 567 2 View

How do you treat cells with H2O2?

Hi, I try to optimise H2O2 dose for HCT116. But cells don't die at 500 uM even after 24 hours. I am suspecting that something is wrong. In forums, people say that they use H2O2 in serum-free...

15 February 2019 5,509 3 View

Interpreation of difference between CCK8 and Cell-Titer Glo?

Hi, Currently I am working with a compound. The compound at 1 uM exhibits no toxicity using CCK8, however according to Cell Titer Glo, the compound at 1 uM kills 80% of cells. Why do I get...

28 May 2018 6,487 3 View

Use of patented technology outside of academia?

Hi all, I would like to initiate a discussion regarding use of patented technology. We all know that there is no restriction to use a patented technology-free to play- in academic research. What...

01 January 1970 7,465 4 View