11 Questions 16 Answers 0 Followers
Questions related from Mert Burak Ozturk
Hi all, I have been trying let B16F10 cells to produce and secrete Melanin upon a-MSH induction. Cells were induced with 50nM, 100nM and 500nM a-MSH in complete DMEM including 0.5mM L-tyrosine...
06 June 2023 2,918 0 View
Hi all, I am looking for some resources showing sponging/trapping mRNA? Most of the papers about sponge function of circRNA show only miRNAs or proteins (RBB)/peptides etc. I need specifically...
06 March 2023 9,993 1 View
Hi all, I wonder whether there is a way to find the original natural product from the modified structure? It is very common to use natural product derivatives in HTS/HCS/in silicon screening to...
01 March 2023 8,208 3 View
Dear all, I am planning to do in vitro transcription from a PCR template. However, I need a poly(A) tailed RNA product. If I add 30 nucleotide poly(A) tail before the in vitro transcription,...
23 May 2022 159 3 View
Dear all, May I know your 384-well plate poly-L-lysine coating protocol for HEK293 cells? Besides, How sensitive is poly-l-lysine to environment conditions such as leaving the stock at room...
29 August 2021 1,301 2 View
Hi all, Currently, I am having difficulty with Gateway LR cloning. Actually with only one gene. For other genes, the technology works almost %100. However, when I tried to transfer HDAC2 into my...
15 July 2021 575 2 View
Dear all, I have been testing some compounds in an assay by using 384-well plate. Compounds are in another plate and transferred into my assay plate (same as HTS) simultaneously. Because I have...
10 July 2021 3,749 3 View
Dear all, Unfortunately, I couldn't find an example on internet, and needed to ask here. I am going to synthetically synthesise my fusion gene along with attL sites, although it will be costly....
04 March 2021 2,133 2 View
Hi, I try to optimise H2O2 dose for HCT116. But cells don't die at 500 uM even after 24 hours. I am suspecting that something is wrong. In forums, people say that they use H2O2 in serum-free...
15 February 2019 5,524 3 View
Hi, Currently I am working with a compound. The compound at 1 uM exhibits no toxicity using CCK8, however according to Cell Titer Glo, the compound at 1 uM kills 80% of cells. Why do I get...
28 May 2018 6,499 3 View
Hi all, I would like to initiate a discussion regarding use of patented technology. We all know that there is no restriction to use a patented technology-free to play- in academic research. What...
01 January 1970 7,477 4 View
