Hi,

I try to optimise H2O2 dose for HCT116. But cells don't die at 500 uM even after 24 hours. I am suspecting that something is wrong. In forums, people say that they use H2O2 in serum-free medium. How do you do H2O2 treatment for long-term treatment and serum-free or with serum?

Thanks

Burak

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