Please look at the following below links which may help you in your analysis:

https://ir.uiowa.edu/cgi/viewcontent.cgi?article=1302&context=etd

https://pubs.acs.org/doi/pdf/10.1021/mp060071q

Thanks

Mert Burak

Treating cells with H2O2 for induced oxidative stress or cell death/damage, you can use 500 - 750 uM H2O2 for 24 h, use H2O2 in medium without FBS.

Thanks Abdoh Taleb