What is the best way of quantitative measurement of bacterial nitrogen fixation?

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Kuldeep Patel

1. Do Acetylene Reduction Assay (ARA): Measuring Nitrogenase Activity (http://www.colorado.edu/eeb/facultysites/barger/Linked%20PDFS/Methods/acetylene%20reduction%20assay.pdf)

2. Grow the bacteria in N-free medium for long time (12-15) days, and estimate the N in media by Kjeldahl Method

Malin Olofsson

I would say that the best way of measuring N-fixation is using stable isotope labelling, check Klawonn et al., 2015 "Simple approach..". Good luck!

Sri Widawati

ya I think u can check Klawonn et al. methods (2015)

Hiep HUU Nguyen

I agree with Dr. Malin Olofsson.