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Questions related from Marimuthu Anandharaj
Actually, i would like to predict the transmembrane region in several membrane proteins. I have tried several online software's including TMpred, MEMSAT, PROTTER, HMMTOP, TMHMM and DAS. I have got...
05 May 2017 6,323 5 View
Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...
12 December 2015 5,411 12 View
I would like to transform my episomal plasmid to Bacillus subtilis, i have many transformation protocol but im confused about which one to choose, if anyone already succeed to use any optimized...
12 December 2015 3,161 1 View
For determining the protein molecular weight, normally SDS-PAGE was used but some researchers also use zymography. im confused with both things. please clarify the difference between these two...
06 June 2014 8,538 6 View
We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...
03 March 2014 6,181 6 View
Why should we use MgCl2 in particular instead of other chemicals for PCR?
03 March 2014 3,263 7 View
I need to identify the gene responsible for production of bacteriocin, and cloned it into another organism but I don't know how to identify the gene from 16S rRNA sequence.
02 February 2014 2,220 2 View
In milk numerous organisms grow well, that's why we pasteurize milk to remove unwanted microbes and their spores. But what is the exact target organism for pasteurization?
02 February 2014 1,111 10 View
The medicinal plants are facing troubles due to anthropological activities, so medicinal plant surveys are helpful to know about the status of particular plants in a particular areas.
01 January 2014 3,543 13 View
I want to know the method which is applied for isolating chemical compounds from plant materials.
01 January 2014 8,644 9 View
I want to know about the metabolomics, if which field it is used...
01 January 2014 6,746 0 View
I want to know what is the need of these two test to select the potential probiotic strains..
01 January 2014 5,917 2 View
There are lots of studies which records the cholesterol reduction by Various Lactobacillus spp. but i want to know is there any researchers showing cholesterol reduction by Lactobacillus oris.
01 January 2014 2,932 1 View
Are there any precursors available for the production of larger amounts of phosphatase enzyme?
01 January 2014 766 1 View
I have to isolate some lactic acid bacteria which includes Weissella spp. For lactobacillus, I used an MRS medium, but I don't think the same media can be used for Weissella spp.
01 January 2014 2,466 3 View
In order to determine the antimicrobial activity of several lactic acid bacteria (Lactobacillus spp.), I want to separate bacteriocins from the culture broth. Can anybody suggest the most...
01 January 2014 4,474 8 View
We extracted secondary metabolite from wild Streptomyces spp. Initially, while running HPLC, peaks was obtained. However, after a few months, the peak disappeared (R.T 5.9-6.4 mins). Any suggestions?
01 January 2014 953 20 View
In earlier periods human milk was believed to be sterile and do not contain any microbial load. Nowadays plenty of research shows the presence of Probiotic microbes in human milk, I want to know...
01 January 2014 6,165 2 View
I want to do TLC for Avermectin assay but I do not know how to do it.
01 January 2014 2,884 2 View
During protein assays, we use TCA to stop the reaction. Why do we use TCA ? Is there any alternative and suitable substance?
12 December 2013 735 0 View
I want to do a keratinase assay using Azokeratin as a substrate, please suggest the protocol of how to do keratinase assay.
12 December 2013 6,292 0 View
Can anybody can explain how to design a primer? Is there any software available for designing primers?
12 December 2013 3,966 27 View
I want to know if it is possible to identify specific enzymes using HPLC?
12 December 2013 9,374 3 View
I want to purify keratinase enzyme from LB broth and I am looking for the best method. Can any one suggest one?
12 December 2013 9,454 8 View
Lots mechanisms were proposed to explain the mechanism of cholesterol reduction, but still there is no clear cut mechanism explained. I want to know any new methods that have been identified?
12 December 2013 4,380 7 View
