I want to know about the metabolomics, if which field it is used...
Actually, i would like to predict the transmembrane region in several membrane proteins. I have tried several online software's including TMpred, MEMSAT, PROTTER, HMMTOP, TMHMM and DAS. I have got...
04 May 2017 6,354 5 View
Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...
11 December 2015 5,466 12 View
I would like to transform my episomal plasmid to Bacillus subtilis, i have many transformation protocol but im confused about which one to choose, if anyone already succeed to use any optimized...
11 December 2015 3,206 1 View
For determining the protein molecular weight, normally SDS-PAGE was used but some researchers also use zymography. im confused with both things. please clarify the difference between these two...
05 June 2014 8,610 6 View
Why should we use MgCl2 in particular instead of other chemicals for PCR?
02 March 2014 3,317 7 View
We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...
02 March 2014 6,224 6 View
I need to identify the gene responsible for production of bacteriocin, and cloned it into another organism but I don't know how to identify the gene from 16S rRNA sequence.
01 February 2014 2,269 2 View
In milk numerous organisms grow well, that's why we pasteurize milk to remove unwanted microbes and their spores. But what is the exact target organism for pasteurization?
01 February 2014 1,168 10 View
We extracted secondary metabolite from wild Streptomyces spp. Initially, while running HPLC, peaks was obtained. However, after a few months, the peak disappeared (R.T 5.9-6.4 mins). Any suggestions?
31 December 2013 1,003 20 View
I want to know the method which is applied for isolating chemical compounds from plant materials.
31 December 2013 8,693 9 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...
17 June 2024 6,124 1 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
How are these exploited in biotechnological applications?
07 April 2024 566 5 View