Which online software is reliable for Protein Transmembrane helix prediction

04 May 2017 6,200 5 View

Double band for a single protein on western blot?

Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...

11 December 2015 5,327 12 View

Can anyone suggest me a best transformation protocol for Bacillus subtilis using electroporation?

11 December 2015 3,054 1 View

What is the difference between Zymography and SDS-PAGE?

For determining the protein molecular weight, normally SDS-PAGE was used but some researchers also use zymography. im confused with both things. please clarify the difference between these two...

05 June 2014 8,429 6 View

Why should we use MgCl2 in particular instead of other chemicals for PCR?

Why should we use MgCl2 in particular instead of other chemicals for PCR?

02 March 2014 3,158 7 View

How to select a correct primer for an unknown organism for PCR?

We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...

02 March 2014 6,089 6 View

Can anybody explain the target organism for pasteurization?

In milk numerous organisms grow well, that's why we pasteurize milk to remove unwanted microbes and their spores. But what is the exact target organism for pasteurization?

01 February 2014 1,002 10 View

How to find a specific gene responsible for particular charecter from the 16S rRNA sequence?

I need to identify the gene responsible for production of bacteriocin, and cloned it into another organism but I don't know how to identify the gene from 16S rRNA sequence.

01 February 2014 2,120 2 View

HPLC Peak has disappeared after one month. Any suggestions as to why?

We extracted secondary metabolite from wild Streptomyces spp. Initially, while running HPLC, peaks was obtained. However, after a few months, the peak disappeared (R.T 5.9-6.4 mins). Any suggestions?

31 December 2013 827 20 View

Is there any record of cholesterol reduction by Lactobacilus oris previously?

There are lots of studies which records the cholesterol reduction by Various Lactobacillus spp. but i want to know is there any researchers showing cholesterol reduction by Lactobacillus oris.

31 December 2013 2,836 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View