12 December 2013 0 6K Report

I want to do a keratinase assay using Azokeratin as a substrate, please suggest the protocol of how to do keratinase assay.

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Which online software is reliable for Protein Transmembrane helix prediction

Actually, i would like to predict the transmembrane region in several membrane proteins. I have tried several online software's including TMpred, MEMSAT, PROTTER, HMMTOP, TMHMM and DAS. I have got...

04 May 2017 6,354 5 View

Double band for a single protein on western blot?

Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...

11 December 2015 5,466 12 View

Can anyone suggest me a best transformation protocol for Bacillus subtilis using electroporation?

I would like to transform my episomal plasmid to Bacillus subtilis, i have many transformation protocol but im confused about which one to choose, if anyone already succeed to use any optimized...

11 December 2015 3,206 1 View

What is the difference between Zymography and SDS-PAGE?

For determining the protein molecular weight, normally SDS-PAGE was used but some researchers also use zymography. im confused with both things. please clarify the difference between these two...

05 June 2014 8,610 6 View

Why should we use MgCl2 in particular instead of other chemicals for PCR?

Why should we use MgCl2 in particular instead of other chemicals for PCR?

02 March 2014 3,317 7 View

How to select a correct primer for an unknown organism for PCR?

We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...

02 March 2014 6,224 6 View

How to find a specific gene responsible for particular charecter from the 16S rRNA sequence?

I need to identify the gene responsible for production of bacteriocin, and cloned it into another organism but I don't know how to identify the gene from 16S rRNA sequence.

01 February 2014 2,269 2 View

Can anybody explain the target organism for pasteurization?

In milk numerous organisms grow well, that's why we pasteurize milk to remove unwanted microbes and their spores. But what is the exact target organism for pasteurization?

01 February 2014 1,168 10 View

HPLC Peak has disappeared after one month. Any suggestions as to why?

We extracted secondary metabolite from wild Streptomyces spp. Initially, while running HPLC, peaks was obtained. However, after a few months, the peak disappeared (R.T 5.9-6.4 mins). Any suggestions?

31 December 2013 1,003 20 View

How can we isolate chemical compounds from medicinal plants?

I want to know the method which is applied for isolating chemical compounds from plant materials.

31 December 2013 8,693 9 View

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