Are there any precursors available for the production of larger amounts of phosphatase enzyme?
for the primary selection According to the literature the Pikovskaya’s agar medium (PVK) was found to be as selectivemedia for the isolation of PSM. pH was maintained at 7.
Actually, i would like to predict the transmembrane region in several membrane proteins. I have tried several online software's including TMpred, MEMSAT, PROTTER, HMMTOP, TMHMM and DAS. I have got...
04 May 2017 6,354 5 View
Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...
11 December 2015 5,466 12 View
I would like to transform my episomal plasmid to Bacillus subtilis, i have many transformation protocol but im confused about which one to choose, if anyone already succeed to use any optimized...
11 December 2015 3,206 1 View
For determining the protein molecular weight, normally SDS-PAGE was used but some researchers also use zymography. im confused with both things. please clarify the difference between these two...
05 June 2014 8,610 6 View
Why should we use MgCl2 in particular instead of other chemicals for PCR?
02 March 2014 3,317 7 View
We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...
02 March 2014 6,224 6 View
I need to identify the gene responsible for production of bacteriocin, and cloned it into another organism but I don't know how to identify the gene from 16S rRNA sequence.
01 February 2014 2,269 2 View
In milk numerous organisms grow well, that's why we pasteurize milk to remove unwanted microbes and their spores. But what is the exact target organism for pasteurization?
01 February 2014 1,168 10 View
We extracted secondary metabolite from wild Streptomyces spp. Initially, while running HPLC, peaks was obtained. However, after a few months, the peak disappeared (R.T 5.9-6.4 mins). Any suggestions?
31 December 2013 1,003 20 View
I want to know the method which is applied for isolating chemical compounds from plant materials.
31 December 2013 8,693 9 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View
I need to know which journals are exempt from publication fees (APC) in the area of microbiology
11 July 2024 6,346 2 View
Hello All, I've been facing difficulty in executing geometry optimization of a sodium polyanionic phosphate (Na4Fe3P4O15) crystal in CASTEP (please find the input and output files attached). When...
07 July 2024 8,626 2 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View