During protein assays, we use TCA to stop the reaction. Why do we use TCA ? Is there any alternative and suitable substance?
04 May 2017 6,200 5 View
Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...
11 December 2015 5,327 12 View
11 December 2015 3,054 1 View
For determining the protein molecular weight, normally SDS-PAGE was used but some researchers also use zymography. im confused with both things. please clarify the difference between these two...
05 June 2014 8,429 6 View
Why should we use MgCl2 in particular instead of other chemicals for PCR?
02 March 2014 3,158 7 View
We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...
02 March 2014 6,089 6 View
In milk numerous organisms grow well, that's why we pasteurize milk to remove unwanted microbes and their spores. But what is the exact target organism for pasteurization?
01 February 2014 1,002 10 View
I need to identify the gene responsible for production of bacteriocin, and cloned it into another organism but I don't know how to identify the gene from 16S rRNA sequence.
01 February 2014 2,120 2 View
We extracted secondary metabolite from wild Streptomyces spp. Initially, while running HPLC, peaks was obtained. However, after a few months, the peak disappeared (R.T 5.9-6.4 mins). Any suggestions?
31 December 2013 827 20 View
There are lots of studies which records the cholesterol reduction by Various Lactobacillus spp. but i want to know is there any researchers showing cholesterol reduction by Lactobacillus oris.
31 December 2013 2,836 1 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View
Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...
22 February 2021 730 2 View
I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples
22 February 2021 1,328 1 View