Hello everyone,

I was wondering if anyone knows another protocol for myelin purification other than the one published by Norton & Poduslo, 1973.

I want to do a phagocytosis assay ex vivo with primary microglia and myelin, and in vivo I want to inject it into the prefrontal cortex of mice to evaluate phagocytosis activity of the microglia from that region.

However, I'm finding a bit difficult to get access to an ultracentrifuge as described in the Norton & Poduslo method.

Any suggestions are well appreciated.

thank you all