Which brand of dissecting tools to use on Drosophila's brain do you recommend the most?

More Marcela Cárdenas-Tueme's questions See All

How can the Stroop Effect be seen in our culture today?

So through social media, advertising, politics and life in general. I know the Stroop Effect is a psychological and biological effect but I am interest to search if, and if yes, it can be present...

13 May 2024 8,371 2 View

Aside from the diagnostic efficacy of the various immunoassays for HDV, do these tests vary in risks or health hazard for the medical technologist?

Pertaining to the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Pan et. al (2023), they discussed the...

09 April 2024 4,044 1 View

What is the recommended search period for a systematic review study on parenting in relation to behavioral problems in childhood?

Considering children between 6 and 18 years of age

03 January 2024 5,076 0 View

Storage of boiled cell lysates with 2xLaemmli buffer?

I am currently in the process of doing a Western Blot with mESCs after an AHA Assay. I added 25uL of 2xLaemmli buffer and 25uL of AHA buffer (50mM Tris HCL) to my pellet. I usually denature my...

05 December 2023 4,334 3 View

I have the challenge of avoiding the degradation of sultamicillin powder for suspension. Any recommendations?

Sultamicillin powder for suspension

14 September 2023 8,015 1 View

Why do I get a white precipitate during my nanosized TiO2 synthesis?

Hello, I´ve been trying to synthesize TiO2 nano-particles via sol-gel by the hydrolysis of titanium isopropoxide without success. Every time I end up with a white suspension that tends to...

22 August 2023 1,970 7 View

Recommended synthesis of Fe3O4@TiO2 Nanoparticles?

Hello, I've been trying to synthesize core-shell Fe3O4@TiO2 nanoparticles with some success. Usually I mix Fe3O4 nanoparticles and TTIP in isopropanol and then dropwise add a 1:3 water+isopropanol...

12 August 2023 1,656 1 View

Hi, I'm looking for the table of E. coli clones for RNAi by feeding for C. elegans worm. Does anyone knows where to find it?

The one in Open Biosystems does not work anymore. Thank you!

02 July 2023 1,187 0 View

How can I achieve large-scale expansion of human mesenchymal stem cells?

Hello! Im working with primary human MSC, I obtained them from adipose tissue, however, I've been trying to expand them unsuccessfully, I use normal T-75 tissue culture flask, DMEM with 15% SFB,...

22 June 2023 6,154 2 View

Can I use over the counter Iodine for μCT Scanning?

The μCT facility I'm at mainly works with fossils. So they don't have any Iodine to stain tissue. I was reading that you can use a 1% Iodine & EtOH solution...

06 June 2023 5,660 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Jonathan M Blagburn

Dupont #5 straight, standard tips. But it is important to additionally sharpen the tips to points that meet exactly with a hard Arkansas stone.

Marcela Cárdenas-Tueme

Thank you!

Nirmala S.V.S.G

Dear Marcela Cárdenas-Tueme

please check the attachment and i do agree with Jonathan M Blagburn

Thank you Nirmala! :)