How to identify and retrieve many (+30) RNASeq datasets (Fasta) under a specific criteria in a practical way?

22 March 2020 3,649 3 View

What have been the contributions of cultural studies to the teaching of English as a second or foreign language?

the idea that intercultural competence develops communicative competences. Worldwide, this relation intercultural-communicative competence has been studied by teachers and researchers (Agudelo,...

01 February 2020 9,056 3 View

How to conduct a Chi-square analysis dealing with two independent groups?

Hello fellow scholars, I would like assistance with a Chi-square analysis dealing with two independent groups. In one research article I was reading, the researcher was studying US attitudes...

25 January 2020 7,039 6 View

How is the Linguistic Colonialism evidenced in the English Textbooks of Multinational Publishing houses in teaching of English as a second language?

The purpose of this question is to examine and compare the concepts of linguistic colonialism and cultural alienation in University textbooks for teaching English as opposed to the theories about...

01 January 2020 1,603 3 View

How to connect beam188 and link180 elements in ANSYS APDL?

I am currently working on the design of a deployable truss structure and I would like to know what is the proper way to connect beam188 and link180 elements in APDL? The diagonals (purple lines)...

07 April 2019 3,152 4 View

What's the amplified gene in the DNA ladder assay for apoptosis detection?

Greettings, I need some guidance. Regarding to the DNA ladder assay for apoptosis detection which is based on electrophoresis analysis, I would like to know what is the gene to be amplified in...

03 April 2019 9,559 4 View

Protein misfolding behaviour?

Hi all, I am working on a tricky protein. I surpassed some initial aggregation behaviour by adding 0.3% CHAPS and 1mM TCEP, buffer in which is happy until the removal of the SUMO tag, then it...

09 December 2018 9,460 3 View

In a Real Time PCR, is a CT of 4.6 (average) enough to determine presence of babesia/anaplasma in a sample?

We are currently trying to identify presence of babesia bovis, babesia bigemina, and anaplasma marginale in DNA isolated from white blood cells. However, the amplification plot values start...

12 November 2018 4,051 3 View

Hi everyone, someone have made real time PCR in tissue fixed ?

for us is very importan the purifiaction and extration of the mRNA in tissue previusly fixed with paraformaldheide, thats possible? or may be we can used other solutions for preserve the sample,...

04 May 2018 8,912 0 View

Does anyone know a clustering algorithm/software that transforms a group of images into an undirected weighted graph?

Hi everyone. I'm currently trying to assess the ability of human volunteers to cluster a set of images into a fixed number of clusters according to perceived visual similarity (the images are...

14 January 2018 8,222 4 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View