What is the best internal reference for qRT-PCR assays of HepG2 cell line under the T3 treatment?

I want to treat the HepG2 cells with either T3 or GC1 (TR-beta1 agonist) for 24 hours. I'm not sure about the internal control for qRT-PCR, in order to accurate normalisation of gene...

20 July 2019 5,877 3 View

How to model the Voids in a rock with zero elastic properties in Abaqus ?

Hello, I am trying to build a model that represents a rock with different minerals in Abaqus to recover the elastic properties like the image. My question is how to define the voids as a...

19 May 2019 6,362 7 View

Finite Element simulation on Abaqus ?

Hello, I am trying to simulate a triaxial test on a rock that has different materials properties. My target is to prove that by increasing the number of cells in the model, I will get more...

17 May 2019 9,772 3 View

Is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

14 January 2019 1,362 3 View

Is it better to measure secreted PCSK9 from medium cultured HepG2 cells or doing western blotting?

I want to treat the HepG2 cells with a drug that down regulates the expression of PCSK9. for assay the result, which method is better?

12 January 2018 7,303 2 View

Does anyone have protocol for lentiviral transduction of HepG2 cell lines?

Hi I need to generate TR-beta1 overexpressed HepG2 cell lines. I could not find any protocol for HepG2 transduction by lentiviral system.

30 December 2017 560 2 View

How can I choose the best plasmid vectors for lentivirus production that have my interest gene?

Hi I want to generate TR-beta overexpressed HepG2 stable cell line. For generating a lentiviral vector that have my interest gene insertion (TR-beta), that have puromycin or blasticidin...

26 December 2017 6,465 3 View

No title

For studying the role of these receptors in my gene of interest regulation in liver I need to have these cell lines

19 September 2017 4,056 1 View

How can I get access to matinspector software for promoter analysis?

every site that I have seen, just is tutorial. Is there matinspector online tool? Is it free?

27 August 2017 4,534 4 View

What are the ethical guidelines for cell line international transportation?

I mean are there any specific codes or regulations in order to govern cell line and genetic materials international transportation?

06 March 2015 6,271 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Which books and review articles describe well the current state of deep reinforcement learning for the optimal control of physical processes?

I am going to treat in a lecture the application of deep reinforcement learning for the optimal control of physical processes. Therefore, I am looking for the best references. Besides the classics...

16 February 2021 1,006 6 View