What is the best internal reference for qRT-PCR assays of HepG2 cell line under the T3 treatment?

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Hello, can anyone help me in isolating, purifying, and identifying native microalgae species?

microalgae cultivation and purification

12 June 2024 2,318 1 View

How does early exposure to a foreign language in infancy and childhood affect the unconscious language acquisition process?

Second language learners often experience immersion in real-life contexts with exposure to cultural nuances, diverse input sources, and strong intrinsic motivation. How about foreign language...

02 May 2024 8,307 2 View

What tests should be done to make a commercial prebiotic supplement in ruminant nutrition?

microbial culture and ....

13 November 2023 6,728 2 View

Can anyone help me and recommend a specialist algae doctor to conduct research??

for animal nutrition/ruminant.goat kids

20 October 2023 1,067 1 View

How to use microalgae in feeding ruminants?

capsule/ fresh/dry and or...

13 October 2023 3,266 1 View

Does anyone know where can i find MCNP code for eye phantom??

is there any MCNP code for eye phantom?

11 April 2023 8,288 2 View

Does anyone know why random number stride warning is appeared while executing input file in MCNP and how to solve it?

I used mesh tally in my program and because of that, the random number stride warning is shown during executing input file in MCNP

21 January 2023 313 4 View

Would you possibly help me to find the difference between depletion and pre-absorption in phage display ?

Hello everyone I am looking for the difference and purpose of depletion and pre-absorption in phage display. How are they different in the procedure, and what is the purpose of it? And when...

14 November 2021 2,872 1 View

Question about the problem solving inventory (Hepener &Peterson,1982)?

As low scores on the problem-solving inventory ( PSI ) indicate high levels of PSC, AAS, PC ( the three factors of the inventory). My questions are: 1-Do low scores mean low arithmetic...

01 April 2021 3,433 3 View

Who can help me on flexible polyurethane foam that does not rise?

what does happen when i make flexible polyurethane foam, it does not enough rise ? it has big bubbles on the top part of the foam. thank you!

23 June 2020 8,688 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View