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Questions related from Kavitha Subramaniam
I have been struggling with B12 (cyanocobalamin) Micro Assay which uses Lactobacillus Leichmannii. I am using USP 171 as the reference method. I'm not quite understood of the procedure. I'm...
19 May 2021 6,771 3 View
Performed a clean room air sampling analysis using a impaction sieve sampler. It compliance with ISO 14698-1. Collected 500L of air at the flow rate of 100 litres/ min for 5 mins. I used TSA...
12 June 2019 5,482 2 View
I am conducting microbiological testing on QC samples recently. I did a test to get Total bacterial count (TBC) on a sample. And I used pour plate method. I am encountering some errors recently....
16 August 2018 6,882 9 View
Is there any alternative serum that can be used instead of rabbit serum in culturing the leptospira bacteria?
16 June 2017 6,405 7 View
Can someone give me an explanation on how to culture Leptospira interrogans? I would appreciate if anyone can send me the journals/articles based on this. Thanks in advance.
13 June 2017 936 5 View
Currently, I'm doing plant extraction using Methanol as the solvent. Now I have 31g of P.longifolia powdered plant material. In order to get the maximum extract, how much proportion of methanol...
07 November 2016 6,653 11 View
I have produced lipase by solid state fermentation. I extract the fungal lipase after 5 days of fermentation. The highest activity was achieved at 0.113(U/gSB) on the 5th day. Sugarcane bagasse...
14 April 2016 1,070 7 View
I need to perform enzymatic transesterification of fish oil for the production EPA-EE (EPA ethyl ester). In case of enzymatic transesterification, is it needed to add KOH or H2SO4? Do wee need to...
30 December 2015 5,989 8 View
For Ethyl Ester identification, the silica plates have to be coated with Argentum nitrate. I need to do the coating by myself. For the detection, the plate has to be spray by sulphuric...
20 December 2015 9,442 4 View
For 'fatty acid ethyl ester extraction from fish viscera' project, I'm using the enzymatic method to extract and hydrolyse the oil. In this case, I'm producing lipase from solid state fermentation...
24 November 2015 4,138 4 View
I'm grinding the fish visceras to make it silage. Antioxidants are needed to prevent the oxidation of the fish due to its high degree of unsaturation. In this case, I'm using hydroquinone,...
18 November 2015 4,883 4 View
For fatty acid ethyl ester (FAEE) identification and quantification on fish oil. Could I get some references on how efficiency and what are the components used in thin layer chromatography? Thanks
27 October 2015 5,939 3 View
I'm currently extracting oil from fish viscera. After the solvent extraction, the fish oil is undergoing transesterification to form EPA ethyl ester. Could anyone provide me some references...
27 October 2015 5,380 4 View
