Is there any alternative serum that can be used instead of rabbit serum in culturing the leptospira bacteria?
Hi Kavitha, this link may be help you
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865830/
Dear Kavitha,
you can use bovine serum for this purpose, the link beloow may help for you
http://www.ivis.org/proceedings/navc/2005/LA/001.pdf?LA=1
As I know only the rabbit serum is used for this purpose but it is possible to try otherwise you may discover something new.
some media you can use
Fletcher (Difco) semisolid medium
Fletcher’s Medium with 5-FU
Stuart (Difco) liquid medium
HI
All researches are used rabbit serum as ingredient in Leptospira media, but the percentage used ranges from 3% to 10% as I know.
Preliminary data from my lab has shown that serovar Ictero has been responding well to 10% FBS.
If you're looking to spur on growth in general, the paper below talks about some of the metrics affecting Leptospira growth.
Article Rapid Isolation and Susceptibility Testing of Leptospira spp...
Dear , look in
Current advances in urban leptospirosis diagnosis
Article · Apr 2017 · Reviews in Medical Microbiology📷 Mir Naser Sayyed Mousavi 📷 Javid Sadeghi 📷 Mohammad Aghazadeh +1 more author...📷 Hossein Samadi Kafil
We are able to propagate Leptospires without serum in HAN medium at 37C, 3% CO2. https://www.nature.com/articles/s41598-020-66526-4
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