For 'fatty acid ethyl ester extraction from fish viscera' project, I'm using the enzymatic method to extract and hydrolyse the oil. In this case, I'm producing lipase from solid state fermentation using fungus. Which is later used for hydrolysis and transesterification as well. The solid state fermentation is carried out for 5 days.
Is there any other method to replace the enzymatic method. I wants to speed up the extraction process? Is there any type catalyst or other method which can be used as alternative.
I would be glad if anyone would help me in this or send me some reference. Thanks in advance.
Look for lipolytic activities by using same fish viscera "packed" on similar devices used for SSF. Try including filamentous fungus.
Solid state fermentation depend upon type of fungi and substrate which you used. It is better to screen the fungi which are capable of hyper producing lipase. If your lipasess are not working in current situation go for immobilization to enhance the activity.
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