13 Questions 2 Answers 0 Followers
Questions related from Kasturee Chakraborty
I would like to observe the biodistribution of nanoparticles inside cells by TEM. I found in some literature that people are using different types of resins for embedding. In one literature I...
17 July 2019 3,543 2 View
Most of the literature shows that Doxorubicin treatment to cancer cells cause a cell cycle arrest in either G1 or G2/M phase. But in my case treating MDA-MB-231 with a Dox nanoparticle caused cell...
14 April 2018 2,794 4 View
I would like to perform an immunofluorescence assay of Erk1 or Erk2 protein in MDA-MB-231 cell line. Is that recommended?
29 March 2018 2,121 3 View
I performed a western blot to check the PARP cleavage after treating MDA-MB-231 cells with drugs for 48 hours but no band was observed whereas in the same blot actin band was observed. What could...
16 October 2017 7,596 5 View
I will be performing Flow cytometry with Annexin V/PI staining to perform apoptosis/necrosis pathway. In this study we would like to put one apoptosis and one necrosis positive control....
26 April 2017 943 1 View
I am working with MDA-MB-231 cell line. I want to check apoptosis after treating with our designed drug. I am planning to do Annexin V PI staining to check apoptosis. I have seen MDA-MB-231...
11 April 2017 3,344 3 View
I will be performing an MTT assay with fibroblast cell line for 48 hrs. What should be the optimal number of cells for seeding.
28 January 2017 591 3 View
Do I need to run RNA in formaldehyde gel with MOPS? Or can it be run in TAE buffer with agarose gel only?
11 January 2017 6,206 4 View
I am looking for the protocol for the preparation of GFP expressing HeLa or MDA-MB-231.
01 December 2016 571 2 View
I have performed MTT assay of Hydrophobic peptides against MDA-MB hrs and -231 cell line at Passage no P12 and P13 at 3 different time points, 24 hrs, 48 hrs and 72 hrs. At 48 hrs, 10 uM...
02 November 2016 3,822 3 View
I am looking for CHD7 silencing siRNA. I found one link in Santa Cruz Biotechnology as CHD7 siRNA (m). I need an opinion for what is the best company to procure this siRNA.
24 July 2016 6,629 3 View
I have taken the optical density of same sample both in a 96 well plate and a cuvette in a microplate reader but obtained different values. And the value obtained in a cuvette is more accurate and...
24 July 2016 2,130 2 View
I will be using a kit CellTitre-Glo Luminescent Cell viability Assay from Promega for performing cellular ATP measurement. The kit describes about the storage conditions where it says it can be...
16 July 2016 3,147 1 View
