Kasturee Chakraborty

9 Questions 2 Answers 0 Followers

Questions related from Kasturee Chakraborty

Can I perform immunofluorescence assay of Erk1 or Erk2?

I would like to perform an immunofluorescence assay of Erk1 or Erk2 protein in MDA-MB-231 cell line. Is that recommended?

29 March 2018 2,119 3 View

I tried to perform a western blot with PARP1 antibody but no band was onserved?

I performed a western blot to check the PARP cleavage after treating MDA-MB-231 cells with drugs for 48 hours but no band was observed whereas in the same blot actin band was observed. What could...

16 October 2017 7,594 5 View

What reagent should I use as positive control for necrosis?

I will be performing Flow cytometry with Annexin V/PI staining to perform apoptosis/necrosis pathway. In this study we would like to put one apoptosis and one necrosis positive control....

26 April 2017 940 1 View

What should be the cell seeding number of fibroblast cells for MTT assay?

I will be performing an MTT assay with fibroblast cell line for 48 hrs. What should be the optimal number of cells for seeding.

28 January 2017 588 3 View

What is protocol for the preparation of MDA-MB-231 or HeLa cells stably expressing GFP?

I am looking for the protocol for the preparation of GFP expressing HeLa or MDA-MB-231.

01 December 2016 568 2 View

What can be the possible reason for variability of cell viability due to the treatment of Hydrophobic drugs at different cell passage of MDA-MB-231?

I have performed MTT assay of Hydrophobic peptides against MDA-MB hrs and -231 cell line at Passage no P12 and P13 at 3 different time points, 24 hrs, 48 hrs and 72 hrs. At 48 hrs, 10 uM...

02 November 2016 3,819 3 View

From where I can procure CDH7 siRNA?

I am looking for CHD7 silencing siRNA. I found one link in Santa Cruz Biotechnology as CHD7 siRNA (m). I need an opinion for what is the best company to procure this siRNA.

24 July 2016 6,625 3 View

Why is the Optical density measured in a plate reader is not as accurate as taken in a cuvette?

I have taken the optical density of same sample both in a 96 well plate and a cuvette in a microplate reader but obtained different values. And the value obtained in a cuvette is more accurate and...

24 July 2016 2,127 2 View

What should be the storage temperature of CellTitre-Glo Luminescent Cell viability Assay for long term?

I will be using a kit CellTitre-Glo Luminescent Cell viability Assay from Promega for performing cellular ATP measurement. The kit describes about the storage conditions where it says it can be...

16 July 2016 3,139 1 View