Cell surface plasma membrane markers that are not present in the mitochondria?

13 October 2023 0 2K Report

I'm investigating a novel target that is present in both cell surface plasma membrane and outer mitochondrial membrane. In skeletal muscle cell line I am running mitochondrial fractionation as I want to focus on the mitochondrial form of this target. However, I need to ensure that the mitochondrial fraction is not contaminated with cell surface proteins, thus is there any protein markers for western blot recommended that are not present in the mitochondria so I can ensure the mitochondrial fraction I have is pure mitochondria.

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