Hello all,
According to my research protocol I could filter the liquid fermentation of a clinical isolate of Aspergillus fumigatus which was grown in SPG broth for 14 days in automatic rotation (180 RPM at room temp). I used a vaccum bomb with a filtration system (filter of 0.2 um) and finally got 280mL free of conidias or hyphas.
And now, I am teasing to evaluate its antimicrobial activity and for that (according to previous reference) I need to EXTRACT the secondary metabolites with ETHYL ACETATE.
I would try with a decantation funnel making 4-5 washes with ethyl acetate (1mL per wash). In my settings I dont have access to MS-GC or any other sophisticated methodologies.
Could anyone help me out with a PROTOCOL OF EXTRACTION please?
I suggest to apply the procedures reported in the following references. Good luck
Nice work. The recommendation by Dr. A GOOD Jawad are ideal. However, let the methodology be modified to meet available resources within your setting.
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