Hi all,
I would like to ask, if two sgRNAs are mixed together at fixed ratio without Cas9 enzyme, say 1:1, how long can they be stored at -20 degrees before use? Should they be used immediately or can be stored upon the next use?
We have to determine easily-extractable glomalin (EEG) and total glomalin (TG) from soil samples, but we have found various contradicting methods and calculations. Is there a trusted method that...
20 October 2020 3,051 3 View
Dear research colleagues, I am currently working on a qualitative research and would like to ask your opinion. I am not sure which qualitative method to use. We conduct semi-structured interviews...
14 April 2020 5,777 17 View
04 March 2020 1,801 16 View
Hey guys! Looking for some statistical advice to anticipate review response. I have done an intervention with 3 groups, 2 of which are in the intervention and 1 that is control. The 2 intervention...
06 September 2019 5,316 3 View
04 March 2019 6,653 6 View
From the signal frequency received by LIGO gravitational wave antennas that is interpreted as originating from a binary neutron star system, we calculate orbital speed of merging components at...
04 February 2018 1,190 42 View
07 October 2017 1,971 22 View
23 September 2017 9,642 50 View
Is the inertia of accelerating masses to be interpreted as due to interaction with the local gravitational potential originating from remote masses of the universe? A gravitational drag experiment...
21 September 2017 8,743 32 View
I want to calculate the single point energy of a molecule in the T1 state using CASSCF. Can anyone post a test file for the above mentioned calculation? The molecule is more stable at S0.
20 June 2017 8,632 3 View
How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...
01 March 2021 2,294 3 View
Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!
01 March 2021 9,574 4 View
I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples
22 February 2021 1,328 1 View
Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...
22 February 2021 3,680 4 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.
21 February 2021 3,081 3 View
I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?
17 February 2021 3,062 4 View
I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...
16 February 2021 8,872 2 View
15 February 2021 911 5 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View