Jing Jin

9 Questions 22 Answers 0 Followers

Questions related from Jing Jin

Detecting gene copy number variation using RT-PCR?

Hi everyone, I am conducting an experiment to detect copy number variation among my gDNA samples using RT-PCR. I have generated standard curve for the genes of my interests and two controls...

24 July 2019 7,000 3 View

RNAseq raw read counts for measuring relative gene expression?

Hi All, I have a raw read count file of RNAseq normalized using TMM in edgeR. In addition to finding DEGs using edgeR, I was wondering if it is valid to compare the number of reads mapped to the...

30 May 2019 7,755 7 View

What's the difference between mitochondrial rDNA and nuclear rDNA?

Hi All, I'm confused by mitochondrial rDNA and nuclear rDNA. Are they the same thing? If not, how different are they DNA sequence wise? Is there ITS region in mitochondrial rDNA? Any comments...

30 March 2018 460 5 View

How can I grow tobacco (roots) on the surface of MS media?

Hi All, I am trying to grow tobacco aseptically on MS media. I would like tobacco roots grow on the surface of the media but not into the media for my further experiments. Just wondering if it is...

17 March 2017 8,888 6 View

How many fungal isolates are needed to present the whole population phenotypically and genotypically in general?

Hi All, I was wondering How many fungal isolates are needed to present the whole population phenotypically and genotypically in general? How can I test the numbers of the isolates used are large...

15 January 2017 8,682 1 View

Can anyone help me interpret the AMOVA test results?

Hi All, I am genotyping 96 isolates (they were collected from different year) with 13 SSR markers. I was hoping to see some kind structure among different years. I did the AMOVA test using R...

31 October 2016 6,975 3 View

How can I analyze sequences with both SNPs and SSRs?

Hi All, I got some sequence data with both simple sequence repeats and single nucleotide polymorphism in it. I was wondering how should I analyze the data? My aim is trying to build a phylogenetic...

18 October 2016 4,386 6 View

What is the requirement of DNA quality in order to do GBS?

Hi All, I'm thinking about to do GBS, but I am not quite sure how good my DNA samples should be, in terms of integrity. I think I have good amount of DNA, but some of my DNA samples may have...

21 September 2016 6,229 6 View

After adding M13 tail to the forward primer, the pair of primer did not work anymore. Has anyone ever had the same situation?

I had 9 pairs of primers added with 4 different M13 tails, two of the 9 pairs primers can not amplify any thing after adding the M13 tails, other 7 pairs worked well. I did not see any small bands...

20 August 2016 7,224 6 View