Jing Jin

5 Questions 13 Answers 0 Followers

Questions related from Jing Jin

Detecting gene copy number variation using RT-PCR?

Hi everyone, I am conducting an experiment to detect copy number variation among my gDNA samples using RT-PCR. I have generated standard curve for the genes of my interests and two controls...

24 July 2019 6,985 3 View

What's the difference between mitochondrial rDNA and nuclear rDNA?

Hi All, I'm confused by mitochondrial rDNA and nuclear rDNA. Are they the same thing? If not, how different are they DNA sequence wise? Is there ITS region in mitochondrial rDNA? Any comments...

30 March 2018 446 5 View

How many fungal isolates are needed to present the whole population phenotypically and genotypically in general?

Hi All, I was wondering How many fungal isolates are needed to present the whole population phenotypically and genotypically in general? How can I test the numbers of the isolates used are large...

15 January 2017 8,665 1 View

What is the requirement of DNA quality in order to do GBS?

Hi All, I'm thinking about to do GBS, but I am not quite sure how good my DNA samples should be, in terms of integrity. I think I have good amount of DNA, but some of my DNA samples may have...

21 September 2016 6,219 6 View

After adding M13 tail to the forward primer, the pair of primer did not work anymore. Has anyone ever had the same situation?

I had 9 pairs of primers added with 4 different M13 tails, two of the 9 pairs primers can not amplify any thing after adding the M13 tails, other 7 pairs worked well. I did not see any small bands...

20 August 2016 7,214 6 View