12 Questions 24 Answers 0 Followers
Questions related from Jing Jin
Hi everyone, I am conducting an experiment to detect copy number variation among my gDNA samples using RT-PCR. I have generated standard curve for the genes of my interests and two controls...
24 July 2019 7,072 3 View
Hi All, I have a raw read count file of RNAseq normalized using TMM in edgeR. In addition to finding DEGs using edgeR, I was wondering if it is valid to compare the number of reads mapped to the...
30 May 2019 7,796 7 View
Hi All, I'm confused by mitochondrial rDNA and nuclear rDNA. Are they the same thing? If not, how different are they DNA sequence wise? Is there ITS region in mitochondrial rDNA? Any comments...
30 March 2018 528 5 View
Hi All, I am trying to grow tobacco aseptically on MS media. I would like tobacco roots grow on the surface of the media but not into the media for my further experiments. Just wondering if it is...
17 March 2017 8,935 6 View
Hi All, I just had a quick question for about genome sequences and gene scan. I was wondering in order to perform gene scan, is whole genome sequence required as the experimental material? Is it...
01 March 2017 6,357 1 View
Hi All, I was wondering How many fungal isolates are needed to present the whole population phenotypically and genotypically in general? How can I test the numbers of the isolates used are large...
15 January 2017 8,738 1 View
Hi All, I am genotyping 96 isolates (they were collected from different year) with 13 SSR markers. I was hoping to see some kind structure among different years. I did the AMOVA test using R...
31 October 2016 7,028 3 View
Hi All, I got some sequence data with both simple sequence repeats and single nucleotide polymorphism in it. I was wondering how should I analyze the data? My aim is trying to build a phylogenetic...
18 October 2016 4,445 6 View
Hi All, I am working on an Oomycete which is a diploid organism, and there are more than 10 genome sequences published, I was wondering if it is possible to do in silico SSR study with published...
05 October 2016 3,963 2 View
Hi All, I'm thinking about to do GBS, but I am not quite sure how good my DNA samples should be, in terms of integrity. I think I have good amount of DNA, but some of my DNA samples may have...
21 September 2016 6,263 6 View
I had 9 pairs of primers added with 4 different M13 tails, two of the 9 pairs primers can not amplify any thing after adding the M13 tails, other 7 pairs worked well. I did not see any small bands...
20 August 2016 7,280 6 View
What's the difference between G protein subunit beta-2-like protein and G protein subunit beta protein? I was interested in G protein subunit beta protein, however, I found the species I'm working...
01 January 1970 8,090 1 View
