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Questions related from Jeremy Polreis
We utilize Shimadzu GC-2010 GC-FIDs and using a supelco SP-2560 column. We notice that we experience a significant retention time shift with injection 1 and gradually the retention time will shift...
21 August 2024 9,469 8 View
We recently started to try a Ninhydrin method to test for amino acid concentration. We are having some issues with the Ninhydrin solution itself. From the method we are trying to accomplish they...
25 September 2023 5,644 0 View
We are having a bit of a debate on weather or not the glass stoppers for our volumetric flasks should be greased. We find sometimes the stoppers on our larger flasks to be dry-locked sometimes...
11 April 2023 8,087 2 View
We are utilizing an aluminum 96well plate system with glass vials due to organic we are utilizing. We need to heat the system to 100C and not let any organics escape. We are having issues with...
10 January 2023 2,994 1 View
We obtained these aluminum 96 well plate systems and are having a hard time finding this red pad that sits on the bottom of the baseplate. Would anyone know who makes or carries this red pad? Or...
01 November 2022 8,646 1 View
We are running some TLC plates to separate lipid components and are utilizing a primuline solutions to stain the plates at the end so the lipids light up under UV. A question was brought up about...
30 July 2021 2,219 3 View
Hello all, We are obtaining some weird LC/MS/MS peaks. We are using a Shimadzu-8050 LC/MS/MS system and are running 0.1% Formic in MeOH and 0.1% Formic in Water for our mobile phase. We are...
11 April 2020 781 0 View
We obtained an Ergosterol standard at 98% pure and made a standard with it. We dissolved the ergosterol in chloroform to obtain a concentration of around 0.5 mg/mL. However, we noticed that when...
14 February 2020 9,295 1 View
We are currently trying to venture into trying to analyze Ricinoleic Acid which is a hydroxy fatty acid. We routinely analyze PUFA/SAT fatty acids. We are having a little trouble with developing...
19 June 2019 5,320 7 View
We are using Shimadzu GC2010 and GC2010 Plus GC-FID with Supelco SP-2560 Capillary column. Lately we we have been running batches of between 30 and 100 we have noticed a significant shift in...
24 April 2017 9,811 15 View
For the fatty acids analysis they will want the short chain fatty acids such as C2:0 as well as the longer chains such as C22:6n3. We have found a few methods for fatty acid extraction however...
01 September 2016 2,542 3 View
Our BF3 lot changed and we are getting junk in our Trans Linoleic Acid peaks and we can not figure out what is causing the junk to occur. Analyzed the peak on GCMS and could not make heads or...
06 June 2016 1,160 11 View
I am looking at being able to measure Folate and Ferritin from RBC on a Clinical Chemistry Autoanalyzer. We currently have a Horiba Pentra C400 to try this on. So far I have only found assay...
01 January 1970 1,581 2 View
A research group wants us to identify and quantify ergosterol in Yeast Cell Pellets. We have recently done regular cell pellets and neuron cell pellets which contained cholesterol. We are having...
01 January 1970 6,510 0 View
