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Questions related from Jan Homolak
I found a bag of old tubes in the lab that look like spin columns(?). There are no labels so I cannot identify them. I spun some water just to see whether they are porous as they seem to have...
29 April 2023 1,179 1 View
I observed an interesting pattern that resembles a "flattening" of the near-UV region of the absorbance spectrum of the rat intestinal mucus in the streptozotocin-induced model of sporadic...
25 August 2022 1,432 1 View
I guess I'm missing some basic knowledge and I may be overlooking something important here... Background: I have a dataset in which animals from 4 different groups (1 control and 3 treatment...
08 May 2021 2,467 3 View
What polymer-HRP secondary ab you had the best results with? How does ImmPRESS compare to others? I'd like to circumvent the biotin-(strept)avidin amplification for multiplexing purposes to be...
22 January 2021 9,982 0 View
Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...
06 January 2021 5,738 1 View
Is there a free software for virtual reality morris water maze tests for humans? Also, are there open arm or other tests in VR format? Thanks! Best, Jan
13 December 2018 320 0 View
Is there a good course on qualitative, semi-quantitative and quantitative analysis of immunohistochemical data on a platform such as Coursera? Thanks.
01 September 2018 2,664 0 View
Hi,For some reason I get much higher c-fos background signal in the rat brain for one animal than for the other (see attached photo). The staining protocol is the same for both specimens, as well...
14 August 2017 5,255 3 View
Hi,Do you have experience with antigen retrieval methods for frozen sections? Can the methods be applied to sections that are already cut (rat brain,35um thick)? Do you have any examples of any...
23 June 2017 1,702 4 View
Hi,I use BDNF immunofluorescent staining in brain and gut. In intestines, it works perfectly, and the staining looks specific according to the literature. However, same protocol with the same...
23 June 2017 5,522 2 View
How would you measure human empathy? What are the recommended questionnaires or test for this purpose? Are they freely available? Thanks
07 June 2017 7,633 10 View
In systemic erythematosus lupus (SLE) anti-dsDNA antibodies can be detected. When we talk about antibodies we usually think about proteins that bind proteins. However, there are also antibodies...
21 May 2017 1,062 2 View
Hi,I want to evaluate neuronal activity after 15 minutes in my slides using immunofluorescence. I can't use c-fos because mRNA levels peak after 15 min, but protein levels seem to be increased...
28 March 2017 1,647 2 View
Does anybody know any undergraduate and graduate student research grants in the field of neuroscience?Thanks
25 March 2017 3,141 2 View
Can BDNF from gut somehow influence the brain? Can you suggest any papers on this topic? Is it rational to think BDNF could go to the brain through blood and surpass liver? Could BDNF alter gut...
16 March 2017 1,823 4 View
Are there any good markers for enteric glia besides SOX10, GFAP and S100b? What are your comments on specificity of GFAP? Thanks
01 March 2017 9,234 3 View
I'm looking for a software that can differentiate sound frequency with a resolution
29 October 2016 6,976 3 View
I am trying to find the best protocol for free-floating frozen IR IHC.
28 July 2016 8,779 2 View
Can you suggest the best antibody for aspartate aminotransferase that is muscle specific? I would like to differentiate liver from muscle AST. Also, what would be the best lab method to use for...
26 April 2016 4,668 1 View
Food-specific immunoglobulin G (IgG) testing is currently not a recognized diagnostic tool for food allergy. Do you think there is theoretical evidence to support IgG food antigens testing in...
01 April 2016 8,536 5 View
Do you know any papers or have any idea how would oral ingestion of galactose affect gut microbiota?
26 March 2016 1,817 4 View
I am trying to find results of standardization for Croatia for BDI-II and BAI.
30 March 2015 9,433 2 View
With some references, if possible,..
03 March 2015 1,119 2 View
What are mechanisms responsible for acute anxiolytic effects of stimulants and how do they fit the most common pathophysiological working models for anxiety?
01 January 1970 8,622 3 View
I'm looking for laboratories working with oxidative stress to test nitrocellulose redox permanganometry (NRP). NRP is a simple method for reductive capacity assessment based on the analysis of the...
01 January 1970 1,681 4 View
