What polymer-HRP secondary ab you had the best results with? How does ImmPRESS compare to others? I'd like to circumvent the biotin-(strept)avidin amplification for multiplexing purposes to be sure I minimized the cross-reactivity problem. I know this can be somewhat minimized with additional A/B blocking and in other ways, but polymer-HRP seems much more straightforward and somewhat faster. Also, any experience with making the polymers yourself (e.g. Article Synthesis of a unique high-performance poly-horseradish pero...

Thanks!