I am planning to conduct pharmacokinetic studies in mice. Considering the availability of low blood volumes per mice, how to collect the samples efficiently in time frames of 5 min, 15 min, 30 min, 1 h, 2h, 4h, 6h, 8h, and 12h? The total blood volume in a mice weighing 25-28 gm is hardly 2 ml.
Dried blood spots (DBS) alongside micro‐analytical techniques are a well vetted solution to the challenges of performing pharmacokinetic studies in such situations. Many, many references available.
Hi Jagtar, it would depend on what is the lower limit of quantification of your analytical method and consequently how much blood you need per sampling time point. Generally for a 25 g mouse the recommendation is not more than 250uL blood in 24h period. I would recommend that if you need 250uL per time point (around 5 drops) then you could make groups of 3-5 mice for each time point. ie. dose 18-45 mice. Alternatively to reduce the number of mice in the study, you could refer literature available for the drug and reduce your timepoints.
Hi
See the papers below my recommendation is a sparse sampling:
Article A comparison of two sparse sampling population pharmacokinet...
Article THE EFFECT OF SPARSE SAMPLING ON THE PREDICTION ERROR IN PHA...
Article Serial and Sparse sampling approaches in single and multiple...
Article Random sparse sampling strategy using stochastic simulation ...
Article Sparse Sampling Design for Therapeutic Drug Monitoring of Se...
Article Sparse sampling approach in rodent toxicokinetic studies to ...
Article Method of AUC Estimation from Sparse Sampling Data
Article Dried Blood Spots and Sparse Sampling: A practical approach ...
Article Optimal sparse pharmacokinetic (PK) sampling strategies (OSS...
Best regards
Tomasz
spare method , there were many studies about that Jagtar Singh