I'm working on carcinogen induced colorectal cancer. I have induced cancer in mice, and the tumors have been induced in the colon as well as in the rectum which is visible externally. I'm also working on human colorectal cancer cell lines in vitro.
I'm confused in this situation, if it is necessary to perform Western blot of drug-treated cell lines, the tumors formed in the colon and the tumors formed in the rectum region.
This sounds like too much work to go with.
Can someone please suggest any way out in this situation !
i mean its never a bad thing to investigate your research from multiple angles, so running a couple of western blots from different samples of cells and or tissue is not that abnormal.
Jagtar Singh if you are restricted for time and funds, just do immunohistochemistry for developed cancers to confirm positive for cancer biomarker, kras for example or a more focused marker you think your carcinogen is inducing/disrupted. Some mechanistic insight always strengthen the argument. I understand WB can be time and funds consuming.
@Rabeah Al-Tamaimi Actually if i will do Western blot of lysate of cell lines, then definitely i have to perform for tumors in the colon, and doing western for tumor present in rectum region of mice will also become a compulsion, otherwise it will invite questions from the reviewer. That's why i was confused what to do in such situation.
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