Why am I not getting bands in 1.8kb PCR for atypical mycobacteria?

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Cheng Shuang huai

The primers is not suitable mycobacteria DNA?

You should compare the primers and mycobacteria genome sequence which probablely been searched from NCBI.

Or rapidly growing mycobacteria 16S-23S rRNA spacer region have mutated, You should design other primers.

Gulnaz Bashir

Thanks Cheng

Lot of work has been done with such primers by other authors previously. can you suggest any other primer sequence to identify atypical mycobacteria.