How to remove water from plant extract??

Hello everyone, I hope these words find you well. I used 80% methanol (solvent) for seaweed's extraction. I have performed rotary evaporator extraction in order to evaporate methanol but there is...

16 October 2020 5,598 8 View

Researchers/ organizations working on gender issues in Vancouver?

Hello everyone, Please reach out if you work on gender studies, gender psychology, social psychology of gender, or gender-based discrimination. You can reach me here or write to me at...

05 August 2020 8,313 7 View

How can we check the real content of calcium in the protein samples?

03 August 2020 4,309 3 View

Capability of MCNP6 in Muon tracking?

Is MCNP6 able to simulate galactic cosmic ray specifically Muons ? if yes does it have any energy or cross section restrictions?

16 June 2020 9,049 3 View

How can I transform dichotomous data into continuous/ Likert?

Hi RG family members, Can someone advise me on how to transform my dichotomous data into continuous variable in SPSS? Best wishes, Gulnaz

08 April 2020 9,849 9 View

Can anyone provide or suggest some reliable articles on second order SEM modelling using SmartPLS.?

Hi everyone. I need some article to understand the second-order SEM modelling using PLS. If anyone can suggest or can share I shall be highly grateful.

03 March 2020 8,605 6 View

Help me construct a research topic on : open drug market and rising spate of drug abuse?

i need assistance on to construct a better research topic on the role of drug market on rising spate of drug abuse.

28 February 2020 8,251 3 View

Can I use an E-Beam patterned Photoresist as a mask for UV photoresist exposure?

Dear Seniors, I am wondering, if I could expose my pattern in E-beam resist (Suggest me one for this purpose) which is on top of UV photoresist (AZ some suitable one). Then I used this written...

21 February 2020 9,650 1 View

Assessment of air microflora in the microbiology Laboratory?

To assess the air microflora in indoor air of laboratories

14 January 2020 2,006 4 View

Which international universities hold the Digital Transformation DBA course?

Hi dears, I want to learn more about Digital Transformation and its impact on Business model, strategy, marketing, agility and ... .I want to apply for online course at universities; is there any...

24 December 2019 3,817 11 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View