I have tried both methods without any success.
Boiling method (Colony PCR) is recommended usually only for Gram negatives, not for gram positives. You can use- QIAamp DNA minikit (Qiagen, Hilden, Germany).
Please try with Bctozol Kit or DNAzol DNA extraction.
I used boiling method for same bacteria .the method was bad DNA extract.the DNA extract from by boiling was counting. sediment
Please used DNA extraction kit
Thank you all.I used GeneJet DNA purification kit (ThermoFisher ) for extraction with excellent results.
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