How good is the boiling method / sonication for DNA extraction from Enterococci?

More Gulnaz Bashir's questions See All

Radial Basis Functions Based Differential Quadrature Method for One Dimensional Heat Equation "required matlab code" and provide code in discription?

I am able to submit the code documents. Although my graph is 1, I would like to be in the 6E-05 graph range. Please, someone correct the error.

08 July 2024 5,770 0 View

When applying the Pooled Mean Group (PMG) estimator, is it necessary to explore alternative estimators: MG and DFE?

When applying the Pooled Mean Group (PMG) estimator, is it necessary and required to explore alternative estimators: Mean Group (MG), and Dynamic Fixed Effect (DFE) as well? Specifically, we use...

24 June 2024 9,621 2 View

EV load Data classification and forecasting?

I want to ask that, is there any method to forecast EV load data recorded at irregular time intervals? I have read some articles related to EV load data forecasting. In these articles authors has...

05 May 2024 4,346 0 View

There are some problems with constructing a tridiagonal matrix A . How to construct this matrix ?

clearvars clc f=@(x)-exp(-5*x)*(9975*sin(100*x)+1000*cos(100*x)); % parameters nx=91; L=1; dx=L/(nx-1); h=dx; x=linspace(0,L,nx); %Boundary conditions u(1,:)=0; u(nx,:)=0; % Multiquadric...

30 April 2024 5,988 0 View

How to implement the compact integrated radial basis function for 1D ,2D PDE and ODE?

MQ RBF use in this paper Integrated diffusion term for 2nd order compare the numerical and exact sol 1D heat eq

23 April 2024 7,115 2 View

What is the best way to collect and prepare sample of African basil and Ashwagandha for molecular analysis?

African basil (leaves) and Ashwagandha (whole plant). i cannot dry in oven nor sun dry

20 March 2024 840 1 View

Timetable scheduling via commutative algebra?

other methods apart from commutative algebra, minimum number of colors, relationship between ideals with edges and nodes,

14 November 2023 9,846 1 View

Why do we do amino acids analysis?

I want to carry Amino acids Analysis on the plant leave Acacia polyacantha both qualitative and quantitative, i want to know the main objective of doing Amino acid Analysis and the main method to...

09 October 2023 3,499 0 View

Please what caused a decreased in quantity of nitrogen adsorbed during BET analysis?

Good day, all, What are the possible reasons for a decrease in the quantity of nitrogen adsorbed during BET analysis for 2 different samples?

08 October 2023 8,681 0 View

Joint research ventures?

Do you think that there should be international collaboration for joint research? If yes, Let's do a great research venture.

23 July 2023 2,594 8 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Dr. Godfred A. Menezes

Boiling method (Colony PCR) is recommended usually only for Gram negatives, not for gram positives. You can use- QIAamp DNA minikit (Qiagen, Hilden, Germany).

Pawel Lisiecki

Please try with Bctozol Kit or DNAzol DNA extraction.

Alaa Kareem Niamah

I used boiling method for same bacteria .the method was bad DNA extract.the DNA extract from by boiling was counting. sediment

Please used DNA extraction kit

Gulnaz Bashir

Thank you all.I used GeneJet DNA purification kit (ThermoFisher ) for extraction with excellent results.