Is an exponential regression coefficient of 0.96 enough for Bacterial Growth?

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CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How do you integrate undergads into research?

I'm passionate about mentoring undergrads into the research process--not just collecting data, lit review, but also analysis. How do others go about working with undergrads in research? How do you...

30 November 2020 3,138 6 View

In Ansys HFSS, how do I replace all instances of a Local Variable within a Design with another variable?

I have created a fairly complex antenna that has a parameterized wire radius (wire_r), however I would like to change the name of the variable to better reflect its purpose (e.g. wire_r_cu). Since...

04 November 2020 7,168 1 View

In Ansys HFSS, is there a method of creating an oscillating (AC) magnetic bias, without resonant peaks, within a non-magnetic substrate, e.g. Air?

A loop sniffer probe antenna for HF/UHF is being developed and we'd like to apply a known, constant across frequency AC B-Field source to the region within the substrate where the probe exists (in...

01 November 2020 5,717 3 View

Is it advisable to dichotomize an independent variable measured using a Likert scale?

I am working on a research project to identify the relationship between sexual esteem and sadistic behavior. Both of my independent and dependent variable have been measured using standard...

16 April 2020 7,062 4 View

Survey Data Anlysis/Testing Support - any help?

Hi - hoping someone might be able to help me please? I've had to totally change my MSc project as a result of COVID and as I can no longer do face to face testing, I have created a survey and...

14 April 2020 6,292 9 View

How is it possible to detect that my UPL probe is expired or does not work?

Hello everybody. I have performed efficiency tests for primers of 12 genes with UPL probes in a Lightcycler 480 to obtain the curves with 4 dilutions of cDNA. I obtained optimal results for 10...

20 January 2020 2,431 1 View

How to preserve endogenous GFP/RFP during IHC?

I have a dual reporter mouse that expresses RFP and GFP under two promoters. I would like to also do IHC on this tissue but the antigen retrieval step (heat) denatures the GFP. Moreover, if I skip...

11 December 2019 403 3 View

Good example of building communication theory using research data?

A colleague and I are working on developing a communication theory. This of course synthesizes prior literature, but we also have survey data we gathered to help us "make the case" for how the...

06 October 2019 1,048 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Does anyone have a copy of the DIBaS dataset?

I am a student from Brunel University London, and I was looking to use the DIBaS dataset for my dissertation. However, recently it has not been accessible via this link:...

26 January 2021 5,185 4 View

Niamh Whelan

Hi yes this is fine, however have you carried this out in triplicate and this is the average? I would always do growth curves at least twice, if the first two are very similar you may skip the third but it is preferable.

Evangelos Stavropoulos

Yes this is fine!

Gregory Guerra Bieberach

thank you very much for your answers... im in the process of making the triplicate :)