hello everyone, i hope you can help me about this troublesome quest:
-I will study 2 conditions with 3 biological replicates (3 samples in control vs 3 samples in treatment) (6 samples in total)
-3 reference genes, and 8 problem genes.
-3 technical replicates for each gene per sample.
this obviously means I wont be able to put all the arrangement in a 96 well qPCR plate.
anyone could refere me to good literature about what considerations should be taken before start?
I have heard about inter run calibrators, other mention use reference genes in both plates, but this is out of possibility since 3 ref genes take 70% of my first plate.
I hope anyone can give me some oppinion and references about this matter.
thank you very much