hello everyone, i hope you can help me about this troublesome quest: 

-I will study 2 conditions with 3 biological replicates (3 samples in control vs 3 samples in treatment)  (6 samples in total) 

-3 reference genes, and 8 problem genes.

-3 technical replicates for each gene per sample.

this obviously means I wont be able to put all the arrangement in a 96 well qPCR plate. 

anyone could refere me to good literature about what considerations should be taken before start? 

I have heard about inter run calibrators, other mention use reference genes in both plates, but this is out of possibility since 3 ref genes take 70% of my first plate. 

I hope anyone can give me some oppinion and references about this matter. 

thank you very much 

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