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Questions related from Gregor Steve
Dear All I am running an salt stress experiment at germination stage using seawater in barley. When we measured Na and K we found that the Na and K had higher concentration n the tolerant plants...
31 March 2023 9,373 3 View
What is the advantages of estimating BLUPs for GWAS studies
05 February 2022 8,106 8 View
Dear All I have SNP markers data for some genotypes and I need to get a table of allele frequency instead of the snps for each locus for example suppose that G1 is A (allele fre = 0.56) and G2...
07 March 2017 3,191 5 View
Dear all In tassel 5. There is an option of creating hybrid genotypes. When I did this, the output is a file in which all sequence between every two genotypes? If I have the phenotypic data...
12 February 2017 1,001 0 View
Do you know any R pakage can deal with unbalanced data set. I have agronomic traits from 7 years with different locations number, different replications number, and different genotypes.
08 February 2017 6,379 7 View
Dear All I have some traits which were scored on bi parental population. I would like to estimate narrow sense heritability. I estimated the broad sense heritability
20 January 2017 1,739 3 View
I have GBS data from wheat and I did SNP calling using tassel. Now, I need to get the flanking sequence around each SNP. thanks in advance
12 December 2016 2,850 5 View
Hi All I exposed some plants to severe salinity stress and cut the plants, then I rewatering again with normal water. Some of these plants started to produce shoots but after 5-6 days, all of...
21 November 2016 4,062 5 View
If you sow one plot (with 50 genotypes at least) and it has one genotype. And you score fusarium head blight by collecting 10 spikes randomly from the plot three times (one time = 10 spikes). Can...
25 August 2016 6,744 11 View
I have 145 genotypes and I would like to analyze them as lattice design. Can I design my experiment with 29 partial blocks with 5 genotyeps in each?
17 May 2016 3,041 4 View
Dear All I need to screen my genotypes with specific SSR marker catching drought and heat tolerance genes. please share your infor if you know.. thanks in advance
04 April 2016 3,646 2 View
Hi all I measure 60 barley genotpes for chlorophyll content using spad CCM200 under three conditions control, drought and heat. I found most of genotypes the chlorophyll content under drought and...
01 April 2016 3,836 14 View
If you have for instance 100 markers and all are mapped in the same linkage group. These markers have a physical positon on chromosomes. So, I found that these markers have positons on different...
24 December 2015 3,344 8 View
What the parentage of missing data allowed for QTL mapping of biparental population?
22 December 2015 6,938 3 View
Hi everybody I have some specific markers for drought tolerance in wheat. Can I use the same marker for drought tolerance in barley? In other words, if I run some experiment to test drought...
08 December 2015 9,062 11 View
In many papers, I found many interesting figures about how authors developed their population. For instance, drawing chromosomes with different color. See attachment
19 February 2015 3,066 3 View
I did genotype 200 lines of barley with 250 SSR and 1200 SNPs. I would like to estimates the gene diversity for each marker.
17 February 2015 499 3 View
I did association analysis using SNP for some traits in medicago. As a result, I got some significant SNP associated with traits e,g Vf_Mt2g086880 . These SNP generated from Medicago. So, I would...
07 February 2015 4,220 8 View
I have 189 SNP markers and 189 line (MAGIC population of 11 parents). I would like to run STRUCTURE and know how many K should I use in my case.
05 February 2015 3,636 4 View
I have 156 SNP markers and I would like to do an association analysis for some traits. And which is better? FDR or Bonferroni corrections? I have read a paper published in Genome and the authors...
04 February 2015 2,037 4 View
my faba bean plants were naturally exposed to frost stress in the field. Some plant did emerge but after exposing to frost they died. If I take a correlation between winter survival rate and 1000...
13 December 2014 7,619 4 View
I am doing association mapping with SSR markers. I have 106 markers when I combined them with phenotypic data I got less QTLs than in the case if I applied minor allele frequency (markers showed...
08 December 2014 8,788 4 View
Hi All Due to the high cost of RNA-seq per sample. Do you think that it will be correct if I bulk three-four biological replicates and send this bulk for RNA-seq?
01 January 1970 1,570 7 View
Hello everyone I have an exon sequence of a gene and I would like to get its cDNA for primer design to test the expression of that gene could you please help to provide any tool that can convert...
01 January 1970 8,926 6 View
Dear All I have scored plant height and spike length in three replication in two years. The analysis of anove was genotypes+replicaiton+years+ R*G+R*Y+R*G*Y I have found high significant...
01 January 1970 8,909 2 View
Dear All I need a reference (proofed study) that report the minimum number of markers for GWAS. Recently, I have read so many papers on GWAS which were published in high-profile international...
01 January 1970 949 4 View
Hello All I am running one-step dPCR in wheat plants. I need to know the concentration of RNA required for dPCR. Should I unify the concentration for all samples such as 10 ng/ul for all...
01 January 1970 1,868 0 View
Hi I have three years data. I would like to estimate BLUEs for GWAS I used TASSEL 5 and PloyqtlR https://rdrr.io/cran/polyqtlR/man/BLUE.html Both of the two software calculated simply the mean...
01 January 1970 4,171 1 View
Dear ALL i need you kind help to get a final concentration of 0.02 mg/ml. I have a mass of dye which its unit is 2 mg. So, this is the following calcualtion are right? I will add 200 ul...
01 January 1970 7,415 0 View
Hi All I am running GWAS in maize population which was tested under water stress. I found that the many significant markers are heterozygous which has three alleles (e.g. C, T, C:T). Do these...
01 January 1970 5,465 1 View
