Hello All
I am running one-step dPCR in wheat plants. I need to know the concentration of RNA required for dPCR.
Should I unify the concentration for all samples such as 10 ng/ul for all samples and go for the reaction?
Currently I am working to defend my thesis, and I ran into a technical question while analyzing my data: Should I conduct an ordinal or a multiple regression? My model is set up such that I have...
20 January 2021 2,104 3 View
The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.
07 January 2021 6,639 10 View
I am looking for the relation between weight evolution (continuous) and a binary factor adjusted for confounders. The regression gives an (adjusted) difference and its CI less than the planned a...
13 December 2020 8,061 3 View
Seeking an intake form for the social science/services field, which may be employed to assess client needs. Additionally, any new instrument for conducting community assessments that can...
29 October 2020 7,612 3 View
Call for speakers/submissions for the online Global Animal Disaster Management Conference (February 2021) now open. This event will bring together world leading experts on animal disaster...
21 September 2020 7,913 5 View
Steven C Stewart (WI, MN) is erroneously listed as Shandra Stewart in many of our joint publications. All dairy related references need to be changed to Steven from Shandra... Thanks!
24 August 2020 7,498 4 View
Hello all, I am convinced that this is bacterial contamination of our Jurkat cell culture, though my team says it isn't. I lack the necessary experience. What do you think about this? The Jurkat...
15 May 2020 8,391 5 View
I am aware of the NEB peptide phage display library, but that does not produce antibodies. Are there any antibody-producing phage libraries commercially available, or perhaps antibody-producing...
12 May 2020 1,026 4 View
Catastrophic reports and publications about the rapid loss of species numbers are becoming increasingly frequent. Furthermore, the biomass of common animal species, such as insects or birds, is...
05 February 2020 7,593 4 View
I read as many old geology papers as I can get access to.
02 February 2020 199 7 View
I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.
03 March 2021 5,997 2 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View