Crystal Digital PCR

Gregor Steve @Gregor-Steve

01 January 1970 0 2K Report

Hello All

I am running one-step dPCR in wheat plants. I need to know the concentration of RNA required for dPCR.

Should I unify the concentration for all samples such as 10 ng/ul for all samples and go for the reaction?

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Gregor Steve's questions See All

Did you know that the NULL-result of the Michelson-Morley-experiment (MMX) has a classical explanation?

The NULL-result of the Michelson-Morley-experiment (MMX) has a theoretical, let’s say geometrical, explanation. You might see it below: If light goes up with 90 degree and comes from that point to...

22 March 2024 3,503 1 View

Why is space 3-dimensional?

Is anyone doing research, investigating reasons for space being 3-dimensional.

17 March 2024 8,243 19 View

How to properly read articles from various fields in the context of interdisciplinary research by a beginning researcher?

Hello! Where can I find comprehensive handbooks or online resources that can help me enhance my skills in reading and analyzing scientific articles across diverse disciplines, from epistemology to...

22 December 2023 8,201 7 View

Can eigenvector equations be understood as being self-referential?

Consider the non-trivial eigenvalue equation Ax = λx. Dividing through by λ gives [A/λ]x = x. Can it be said that any vector x that solves this equation is a fixed-point in the vector space? And...

22 November 2023 3,425 3 View

What Qualitative method can I use to study the potential of a phenomenon?

If I want to assess the potential threat of cyber terrorism to the aviation industry of a state (consider 5 aspects: knowledge, awareness, vulnerabilities, response and impact) and have the...

17 November 2023 1,099 3 View

What Qualitative method can I use to study the potential of a phenomenon?

If I want to assess the potential threat of cyber terrorism to the aviation industry of a state (consider 5 aspects: knowledge, awareness, vulnerabilities, response and impact) and have the...

17 November 2023 5,790 3 View

Define creativity, innovation, organizational creativity and leadership?

definitions, steps in creative process and levels of leadership

12 November 2023 4,492 4 View

Does anyone have a culture of Monascus purpureus culture?

I only found it on the atta but too high for someone who has retired. instead of buying buying red yeast supplements and not know the % of Monacolin K, I'd like to grow it in liquid culture,...

14 August 2023 2,815 1 View

How is the Universe expanding proportionally to the space distance from "here" and shrinking proportionally to time distance from "now" ?

This looks as a paradox of the Big Bang theory. The further we look in space the Universe is getting larger and larger. But, at the same time, the further we look in space we are also traveling...

19 July 2023 6,494 4 View

Where can I buy Computer time that has a SPICE simulator with 128 Bit floating point?

I have been working with ngSpice circuit simulator for a couple of months now and have had to give up because of bad (noisy and glitchy) simulation results. I don't feel like I can trust any of...

17 May 2023 4,088 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View