Hi Steve,

You may try to PCR barley with your wheat markers and see what you got. The chance is weak but it worth being tried as it is not too much labor and cost.

Good luck,

Pierre

I you get a parallelism in drought tolerance of wheat and Barley, then OK. But such a situation has remote possibility because two genera have different physiological responses and their genetic make is also different.

Dear Pierre:

Thank you for your answer. I think I will not loss any thing if I tired in a matter of gaining experience...  

Prashant:

Thanks. do you publish this work? drought tolerance in barley and wheat together... I appreciate if you send me this work

Hi

it depends on what the markers are for, some traits are generic for drought tolerance, others not , there are species differences as pointed above; many traits also work together or in a hierarchy, so it is hard to say. Try running the markers and see what you get... 

HI Gregor

In many studies, Germplasm analysis to study genetic diversity is another important area in which a lot of efforts have been put in. Fingerprinting of crops like rice, wheat chickpea, pigeonpea, pearlmillet, etc. is being carried out extensively. This information has potential in strategic planning of future breeding towards crop sustainability. Apart from use of molecular markers in crop plants, efforts are also underway in other horticultural plants. Early identification of sex in dioecious papaya using molecular marker.

check attachment 

http://www.iisc.ernet.in/currsci/jul25/articles15.htm

to some extent it can used but with less results , so researcher may do that when the they first time applying the technique for new crop.

I think this is not feasible. There are not many examples of using markers developed in one crop in another. In fact, even markers have the tendency to be lost iif you want to use them in a different genomic structure (i.e., species relative, breeding populations) within the same species. On top, drought response involves many biological events which create tolerance---many genes are expressed and structural changes at the cellular/physiological level. I would ideal to develop your own  set of markers for your materials.

Most of the molecular markers for abiotic stress tolerance are common among similar species belong to same family like wheat, barley and oats. or example a marker like Transcription factor (TF)  or SOD like scavenging factors are common in many species. Try the marker by using PCR or qPCR for consistent over expression under drought conditions and use as a selection tool in barley.

It depends on the markers you have, because usually some genes involved in the pathway of drought tolerance are conserved in plants, cells behaving almost in the same way under that kind of stress. However, specific transcription factors or regulators can be slightly different in barley or wheat: you can simply try which ones work in PCR on your cereal. It's the first step before studying QTL associations.    

No, not possible. In principle, a marker is not necessarily designed within the gene involved. A not tightly linked marker may be 1000 bp upstream for instance. Lets assume the marker is tightly linked (i.e., designed on the gene involved), it may be designed around a SNP or InDel that distinguishes between Wheat A and Wheat B. This will tell you nothing about a possible difference between Barley A and Barley B. 

I'd recommend you find out how these markers were designed; are the genes of interest in Wheat described? If so, try to amplify and compare these genes in your Barley races and design new markers.

ps. what are the ploidy levels?