I was looking for antibiotic genes/clusters in a recently sequenced strain of Streptomyces. I was wondering if the presence of 4'-demethylrebeccamycin gene suggested rebeccamycin synthesis ?
I was looking for the most recent antibiotic to be approved for clinical use that has a significantly different structure from previous antibiotics. Preferrably sourced from the natural...
04 October 2023 3,314 3 View
If you can change your lifestyle/behaviour, how soon can these changes be detected in the epigenome? How long (estimate) would this change be detected in the majority of cells? Can this change...
21 July 2023 3,933 1 View
Hi there - hoping to get an SME on self-regulation theory who might be able to suggest the most valid scale that is a reasonable length. There seem to be many synonymous constructs, from...
20 September 2022 9,134 0 View
I am looking for the WGS of a variety of Chickens (Gallus gallus) such as Rode Island Red or White Leghorn. I realize there might not be a great diversity between these breeds but I would like to...
21 April 2022 5,231 1 View
I am trying to identify some small molecules in a bacterial supernatant extract. I would like to remove proteins to make mass spectrometry easier. I have tried protein crash with ACN but the...
03 March 2021 4,819 4 View
I am interested in identifying sex-specific survival rates. I have a long-term dataset of individually marked birds from one breeding colony. Of these, I am able to genetically sex a subset....
14 January 2021 7,549 4 View
Hi all I use psychopy 3 to show mice a visual stimulus on an LCD screen while preforming acute extracellular recordings in vivo. I understand that one should use a spherical wrap to the stimulus...
22 November 2020 8,103 2 View
How do I find out what publications cited my article? Ronald Quinn
11 October 2020 1,021 4 View
Hi everyone While the anesthetized mouse is held with stereotaxis ear bars and bite bar, we record LFP signals from the visual cortex in response to gratings presented on a 5' screen in front of...
21 June 2020 417 0 View
Hi, I am performing GST purification, however, I am getting poor signal with my GST antibody at the recommended dilutions -...
11 June 2020 9,950 1 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View