I was looking for antibiotic genes/clusters in a recently sequenced strain of Streptomyces. I was wondering if the presence of 4'-demethylrebeccamycin gene suggested rebeccamycin synthesis ?
Hi all I use psychopy 3 to show mice a visual stimulus on an LCD screen while preforming acute extracellular recordings in vivo. I understand that one should use a spherical wrap to the stimulus...
22 November 2020 7,905 2 View
How do I find out what publications cited my article? Ronald Quinn
11 October 2020 762 4 View
Hi, I am performing GST purification, however, I am getting poor signal with my GST antibody at the recommended dilutions -...
11 June 2020 9,769 1 View
Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...
30 January 2020 9,060 2 View
Substance use disorders have been and will continue to be problematic for our society, what approaches can we utilize that will offer the greatest chance of success of abstinence and sobriety? It...
14 October 2019 9,528 1 View
My topics and questions are only in the early stages and haphazard. It will be a literature review. So far I am thinking of the effects of sedation breaks in adult ICU patients. Early...
21 February 2019 2,819 9 View
We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?
15 January 2019 2,482 3 View
I performed some comparitive proteomics between planktonic and biofilm membrane proteomes using tandem mass tags. The mass spectrometry generated HCID and CID data . I now need to identify all...
13 August 2018 5,971 3 View
I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.
03 August 2018 2,101 2 View
I had previously made a series of (modified SFM) agars at high alkaline pH 8----->13.6. I did not measure the pH of the resulting solidified agar because it just seemed a bit awkward and I...
07 March 2018 1,160 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View