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Questions related from Frank Liu
After one harvests a mouse spleen and isolates the NK cells (through a commercial kit, for example) roughly how many NK cells can one expect to be left with? If anyone has rough numbers they can...
29 January 2022 5,445 2 View
My goal is to establish an in-vitro culture of mouse splenocytes that are lacking in either CD4 T cells or CD8 T cells. I tried doing this in the past by administering IP injections of anti-CD4...
14 January 2022 5,648 3 View
Hello all, I recently treated telomerase-inhibited A549 cells with 5 uM RITA, a p53 activator drug, and measured cell viability using the MTT assay. While wild type A549 experienced an expected...
04 March 2018 3,129 2 View
Hi all, When I am conducting PCR in order to amplify a certain DNA segment for subsequent Sanger sequencing, would using the conventional DreamTaq Green Mix from Thermo Fisher work fine? Or...
13 February 2018 7,507 3 View
Hello all, I am performing telomerase inhibition experiments that will result in replicative senescence in cancer cell culture. Can anyone recommend assays to use to measure replicative...
05 February 2018 8,926 2 View
I treated wild type A549 cells with 10 nM RITA, a p53 activator drug, and the cell viability increased 18.7% in a statistically significant manner (p
10 January 2018 8,041 1 View
Trying to save cost here...can I grow tissue culture in a non-CO2 incubator as long as the media is supplemented with HEPES as a buffer instead? Thanks.
31 July 2017 2,380 3 View
I'm working with a DNA purification kit in order to purify and collect plasmid DNA from E. coli bacteria. The manufacturer's instructions say to resuspend a bacterial pellet formed after...
23 July 2017 5,760 4 View
Will the Cas-9 enzyme still be able to recognize and induce double strand breaks on condensed or highly condensed regions of DNA within eukaryotic cells?
22 June 2017 480 2 View
I am aware that there are two different methods for double strand break repair (non-homologous end joining or homology directed repair), but what I wanted to know is if the repair mechanism...
19 June 2017 5,685 3 View
