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Questions related from Florence Mia
We mainly work with primary cells and the only system that has successfully been use for KO or O/E experiments has been the use of lentiviral systems. However, our lab is abandoning the use of...
06 July 2021 7,259 3 View
I am using a lentiviral TET-ON system for conditional overexpression of my gene of interest in primary kidney cancer cells. I use viral load from transfection of HEK293T cells after 48 and 72...
17 November 2020 2,646 1 View
I am trying to express my gene of interest using a Tetracycline inducible vector. Although I need my gene of interest of be off and only be turned on for a short period of time when needed, I only...
08 July 2017 4,092 19 View
I am having some issues with primer optimization for qPCR. Typically, I first test various concentrations of primers to figure out which concentrations gives the best melting curves and the lowest...
18 January 2017 10,017 2 View
I am performing ChIP on a DNA repair factor in budding yeast. We are interested in looking at the enrichment of this factor in WT and some mutant strains. And I am using a strain that can induce a...
31 August 2016 1,902 2 View
I am trying an experiment in which I measure the NHEJ efficiency of a mutant yeast strain and compare to wild-type to analyze whether the mutation affects the NHEJ process. I take a low copy...
17 December 2015 2,674 3 View
I have biotin-labeled substrates and I want to visualize them under the microscope. I'm thinking of immobilizing streptavidin on slides or coverslips and then incubate them with my substrates. I'm...
30 October 2014 3,565 6 View
Is it because of heterogeneity in the extent or form of glycosylation or does it have another reason?
14 August 2014 972 19 View
Most therapeutic strategies use inhibitors to target a specific function. Are there examples where a drug induces upregulation of a gene? I'm specifically interested in examples in fungi.
22 June 2014 3,297 2 View
There are some examples of actin-independent endocytic pathways in mammalian cells. What about fungi? Are there any studies on fungi?
06 June 2014 322 2 View
When designing inhibitors for an enzyme, is its sub-cellular localization an important factor? Does it matter where the enzyme functions (e.g at cell surface vs. intracellular compartments)?...
03 June 2014 5,311 1 View
Are there known pathways to transport proteins from vacuole to the plasma membrane in budding yeast?
27 May 2014 9,538 1 View
I have a probe tagged with mVenus and would like to use it as a donor in a FRET experiment to detect interaction between the probe and a histone modification. I'm planning on using an antibody to...
18 April 2014 1,860 1 View
