What is the reason for change in mRNA level of specific gene but not change in protein level?

With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...

07 August 2017 1,964 7 View

How can point mutation in a protein affect amount of other protein in cells?

It has shown that about gene knock out in mice'brain, the amount of other protein can be affected (Maybe in mRNA level or protein stability). I want to know whether point mutation can lead to the...

04 May 2017 3,735 4 View

Have anybody experience in taking special GRE in biochemistry?

I'm looking for a set of books that I can read in preparation for the GRE Subject Test In Biochemistry, Cell And Molecular Biology

08 September 2015 8,014 1 View

Can anybody introduce books in field of oncology?

I want to know oncology books that is comprehensive. I want to know from beginner to advanced. I need materials that is useful for molecular medicine major.

06 July 2015 8,964 3 View

Does rigidity structure of enzyme always lead to decrease enzyme activity?

Article show that rigidity affects enzyme activity and high rigidity causes low activity. Is there any exceptions? How does increasing rigidity cause high activity?

01 February 2015 9,685 11 View

What is the effect of immidazole on activity and stability of enzyme?

When enzymes were eluted with high concentration immidazole, some of them lost their activity in 2 days. I want to know how immidazole affect enzyme or other protein. I can't find article about that.

31 December 2014 8,559 7 View

How does the removal of a small domain from an enzyme affect the optimum PH of activity?

my enzyme is cellulase. It has two domains( small domain and catalytic domain). small domain has a few hydrophobic interaction with catalytic domain. I determined PH optimum for truncated and...

11 December 2014 8,513 4 View

How can I assay the aggregation of my enzyme?

I removed small domains from my enzyme. I want to know which one (truncated or wild-type) is more likely to aggregate at different times at 65 centigrade. Can you give me a strategy to go about...

11 December 2014 5,926 9 View

Why do truncated and wild-type enzyme have different colors in bradford assay with the same sharpness in SDS-PAGE?

I remove 85 aa from my enzyme(cellulase). I purified enzymes(truncated and wild-type). After dialysis, I run 20 microliter of them for SDS-PAGE. Both of them have the same sharpness on gel, but...

10 November 2014 7,843 4 View

Why did my protein elute with wash buffer (even 10 mM imidazole)?

I can't purify my protein with the ni-agarose column. The PH wash and elution buffer is ok but all of my protein eluted in the wash buffer. What is my problem?

09 October 2014 981 17 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View