Dear researchers,
The attached images are from the leaves of transformed plants by empty vector pCAMBIA1302 (GFP),
so the images are ok and can use for a manuscript?
Your comments are appreciated
Thanks
Dr- Hafiz Muhammad Rizwan
1. At least some parts of the leaves have GFP glowing. And the color is right (compared to those posted in another thread).
2. Why does GFP only glow on some areas (indicated by yellow arrow), not on other parts of the leaf (indicated with red circle)?
3. It is only 'empty vector' transformation. It is usually serve as a 'control'. You need a 'Gene-of-interest' in the binary vector as an 'experimental' group for publication. You might be able to get by (get published) if this is the first successful genetic transformation case in your plant species.
Yuan-Yeu Yau 2. Why does GFP only glow on some areas (ex. yellow arrow), not on other parts of the leaf (red circle)?
It should glow on the whole leave area?
Dr- Hafiz Muhammad Rizwan
1. Should be. Don't you think so? The promoter to drive eGFP is 35S promoter. Unless, 'gene-silencing' occurs at some parts of the leaf....
2. Is this the first successful genetic transformation case in that plant species you are using?
@Yuan
1.Yes should be,
2.yes first time trying
what about the following images. i tried again
Yuan-Yeu Yau Yes sir, it should be,
Yes in passion fruit first time trying
The following attached images were shooted by mobile from the PC screen,
So empty vector will be like this? as attached images, I tried again
Dr- Hafiz Muhammad Rizwan
Yes, that looks GFP all over the leaves. What is the difference between A and B (slide 4)?
Dr- Hafiz Muhammad Rizwan
Ok. Thanks. How long does it take for passion fruit to flower? Just curious.
Yuan-Yeu Yau It depends on the technologies and conditions, around > 1year to start flowering
Some plants has autofluroscence properties that gives GFP like fluroscence. And dead cells also gives GFP like fluroscence. If you want to confirm this fluroscence are GFP signals are not, you should check either it colocalised with chlorophyll or not.
Most Tanziman Ara Thank you Dr for your answer, so how to check either it colocalised with chlorophyll? can you please share the protocol
Thanks
- Badges
- Science topic
- Similar topics
- Vectorization