Anyone has an experience extracting proteins from rectal swab samples?

I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...

02 February 2021 3,622 3 View

How do you check the occupied d-orbitals in VASP?

I am running VASP calculations involving transition metal atoms in which there are multiple unpaired electrons in the d-orbitals but I am not sure how to check the individual d-orbitals (ex:...

24 August 2020 1,947 2 View

Can you check spin multiplicity on individual atoms in VASP?

I am doing VASP calculations with a bulk triclinic lattice system involving transition metals Cr and V. I know that you can check the spin for the entire system with the OSZICAR file from VASP but...

16 July 2020 9,052 3 View

How to make a scatter plot for many variables and 2 groups in one graph?

I have 2 groups and have 7 variables. I would like to make a stacked scatter graph to present it all in one graph. Is it possible and how?

13 July 2020 2,145 3 View

Beta value calculation of absolute intracellular calcium concentrations?

Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...

24 March 2020 7,974 1 View

Workfunction from LOCPOT?

Does anyone know how to derive the work function from the LOCPOT file from vasp?

24 March 2020 851 5 View

I have 2 groups to compare by removing the effects of age and the data is not normal. How do I do so in SPSS?

The 2 groups are parents and their children. The data is not normal. Would like the comparison without the effects of age.

21 February 2020 7,714 4 View

Hi, I have child-mother data on a quantitative trait and need to know how to calculate their heritability?

I have done a spearman correlation as the data is not normally distributed and now would want heritability index...am I correct in looking at heritability with this data? If yes, how do I go about?

14 February 2020 9,160 3 View

Experimental method for determination of C-H (homolytic) BDE in organic compounds?

Hello Everyone, We want to measure the C-H (homolytic) bond dissociation energy of some organic compounds. Any practically feasible tool in the common analytical laboratory that can give us the...

13 February 2020 7,588 2 View

How to decide on which MDR strain (GN bacterium) to buy from ATCC?

I want to purchase well characterized MDR strains of a Gram-negative bacterium from ATCC to look at modes of resistance. I am also going through literature to see if people have purchased a...

05 June 2019 7,721 1 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

Are there any fast-acting, long-term fixatives compatible with FISH?

I'm looking for a fixative that works quickly but you can safely store cells in for a longer period (i.e. a few hours). Most importantly it must be able to fix chromatin well and be compatible...

03 February 2021 7,659 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View