24 Questions 38 Answers 0 Followers
Questions related from Cristina Andrés Barrao
Bacteria are able to produce different pigments, with have different functions. But besides carotenoids (red-orange-yellow colored), what other compounds may a bacteria produce that makes it...
02 February 2018 1,888 5 View
Hello everyone, Please, can anyone address me to any source where I can find the different starting codons for non coding rrnas in prokaryotes? I know the known translation starting codons for...
07 July 2017 5,306 1 View
Dear all, I normally apply GTG5 fingerprinting to genotype a set of bacteria in our group collection. I was being successful from the beginning, but now the reactions do not work, just giving a...
05 May 2017 1,208 6 View
Hello everyone, For bacterial taxonomy based on the 16S rRNA gene, it is currently accepted the similarity cutoff of
02 February 2017 9,796 3 View
Dear all, I am trying to determine the efficiency of a set of primers for qPCR. Being my very first qPCR... it doesn't seem too good to me. Please, seeing the image attached, does anyone have an...
10 October 2016 9,900 5 View
Hello, I am analyzing a set of genomes that our group have sequenced and annotated. Trying to perform GO enrichment from a set of genes that were observed up and down regulated (from RNA-Seq...
09 September 2016 3,396 7 View
Hello, To those who have experience in using flow cytometry for counting bacteria (anexic cultures), I would like to know what is the detection limit. I just had a kind of argument with a...
06 June 2016 3,596 2 View
Hello, I am working with a Gram- bacteria that has been isolated form soil, tentatively identified as Enterobacter sp. I am infecting seedlings and after germination and few days of plant growth,...
05 May 2016 2,224 6 View
I have obtained diverse opinions from my close environment about what is better for your resume between the publication of a review or a book chapter. Some people argue a book chapter is better...
01 January 2016 6,853 1 View
Hi everyone, To those who are studying gene differential expression by using RNA-seq, can you share with me the protocol you follow for validation of candidates genes by RT-qPCR. I mean the PCR...
10 October 2014 9,832 1 View
Hi again, I know most people, expert bioinformaticians, use DESeq and EdgeR packages in RBioconductor to analyze differential expression from RNA-Seq data… Unfortunatelly, I'm experimentalist and...
10 October 2014 4,891 4 View
Hi everyone, Experimentalist trying to get introduced to some basic bioinformatics, I've recently started to use R Bioconductor, but getting quite lost yet.I'm would like to use this new skills to...
10 October 2014 5,754 3 View
Hi everyone, I've used MAUVE before to work with a set of bacterial genomes and had no problems. Progressive alignment was performed by using genbank (.gbk) files as input and I obtained a nice...
07 July 2014 7,580 4 View
Hello every one. Hope you can help me. The task seems to be rather easy. I have a set of bacterial draft genomes in .gbk format and I want to used them as input into KEGG Automatic Annotation...
07 July 2014 5,800 3 View
I'm trying iPath 2.0, but I found some inconsistencies. It is supposed to use the KEEG database and my organisms is contained in this database, and I'm using a set of orthologous groups that have...
06 June 2014 4,865 12 View
After starting to work a little while ago with RNA in prokaryotes in order to perform RNA-Seq analysis, I finally arrived to the moment of the bioinformatic analysis of the obtained reads. I...
05 May 2014 5,373 3 View
Dealing for the first time with RNA, I'm trying to extract several samples of gram negative bacteria in order to perform a RNA-seq analysis afterwards. The problem is that I want to analyze...
02 February 2014 6,364 10 View
I'm dealing for the first time with RNA, with the final aim of performing RNA-seq analysis to estimate the differentially expressed genes of a gram negative bacteria during a certain process....
02 February 2014 8,511 12 View
I've started to analyze organic acids by using HPLC a few months ago. For this purpose, I was trained by a master student in the utilization of the HPLC device, and the protocol he transferred me...
01 January 2014 3,913 13 View
I need to use a certain volume of 14.4 M "commercial solution" of 2-mercaptoethanol. The problem is that in the lab (I recently joined this lab) they only have a bottle purchased from WAKO, Japan,...
10 October 2013 8,598 8 View
I'm trying to find any commercial supplier of the following chemical compound: 2,5-diketo-D-gluconic acid, acid solution or salt. I found strange that in a reference from 1984 they use commercial...
10 October 2013 3,898 2 View
I already read a lot of discussions concerning this matter, and realized the diversity of techniques used to prepare this bacteria glycerol stocks. Some procedures follow specific steps due to the...
10 October 2013 5,303 21 View
I'm using the RiboPure-Bacteria (Ambion) for extracting total RNA from Gram negative bacteria that have been harvested and the pellet resuspended in RNALater reagent, but using a slightly modified...
08 August 2013 4,656 21 View
I'm starting a new project relating to the RNA of gram- bacteria. I've performed a few extractions of total RNA using the RiboPure kit from cells stabilized in RNALater solution, and all seemed to...
08 August 2013 3,708 20 View
