What can bacteria synthesize, giving them yellow color, that are not carotenoids?

Bacteria are able to produce different pigments, with have different functions. But besides carotenoids (red-orange-yellow colored), what other compounds may a bacteria produce that makes it...

01 February 2018 1,834 5 View

What are the different starting codon for non coding rnas (rRNAs, tRNAs, ncRNAs) in prokaryotes?

Hello everyone, Please, can anyone address me to any source where I can find the different starting codons for non coding rrnas in prokaryotes? I know the known translation starting codons for...

06 July 2017 5,253 1 View

GTG5 genotyping troubleshooting?

Dear all, I normally apply GTG5 fingerprinting to genotype a set of bacteria in our group collection. I was being successful from the beginning, but now the reactions do not work, just giving a...

04 May 2017 1,114 6 View

What is the similarity cutoff for delineation of bacteria genus and species based on MLSA?

01 February 2017 9,744 3 View

How can I improve my qPCR reaction?

Dear all, I am trying to determine the efficiency of a set of primers for qPCR. Being my very first qPCR... it doesn't seem too good to me. Please, seeing the image attached, does anyone have an...

09 October 2016 9,851 5 View

How to do GO enrichment for genomes not public in any database?

Hello, I am analyzing a set of genomes that our group have sequenced and annotated. Trying to perform GO enrichment from a set of genes that were observed up and down regulated (from RNA-Seq...

08 September 2016 3,342 7 View

What is the detection limit for counting anexic bacterial cultures by using flow citometry?

Hello, To those who have experience in using flow cytometry for counting bacteria (anexic cultures), I would like to know what is the detection limit. I just had a kind of argument with a...

05 June 2016 3,547 2 View

Why bacteria do not precipitate after centrifugation?

04 May 2016 2,148 6 View

What is better, review or book chapter?

I have obtained diverse opinions from my close environment about what is better for your resume between the publication of a review or a book chapter. Some people argue a book chapter is better...

31 December 2015 6,800 1 View

How can I convert a Genbank file (.gbk) into a .ffn file using R Bioconductor?

Hi everyone, Experimentalist trying to get introduced to some basic bioinformatics, I've recently started to use R Bioconductor, but getting quite lost yet.I'm would like to use this new skills to...

09 October 2014 5,700 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View