I was wondering if bathing with Apyrase would affect amount of intracellular ATPs as well as extrecellular ones.
Can I say Apyrase only affects extracellular ATP-ADP ratio?
If it does, I expect an increase in the rate of respiration. That is always a worthwhile control any way.
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Hello, Dear! Generally, I used CHARMM36 + cgenff force field for simulaiton. But,, due to certain parts of lignad, force field cannot be generated using Cgenff. Can I use different force field...
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I have a cathode electrode (5 x 5cm) on the femoral triangle and an anode electrode (5 x 10cm) on the gluteal fold. We are doing repeated high-intensity cycling followed by repeated Wingates. We...
07 July 2024 833 1 View
I wonder the tendency of coefficient of thermal expansion (CTE) of polymer which has different chain structure. In my opinion, CTE will be higher when the polymer materials has high interaction...
30 June 2024 570 5 View
To whom it may concern, I'm working on optimizing the molecule using gaussian 16. However, it is suffering from the problem of convergence failure. I attempted optimization several times, but...
30 May 2024 6,373 3 View
I want to experiment with microbial control through chlorine disinfection. What I have on hand is 5% sodium hypochlorite solution. I want to set the chlorine injection concentration to 1, 3, 5,...
01 May 2024 3,741 0 View
Greetings to science community, I am operating Kluyveromyces marxianus yeast and want to generate haploid cells from diploid cells. However after 4 day since dissection, I found no growth of...
09 March 2024 8,360 0 View
can anyone give me advise about charge transfer transition of transition metal and rare earth element? and usually people see the FL and absorption, emission spectra of those materials in glass,...
08 March 2024 7,916 1 View
H1781 cells are shown to tend to clump each other. H1781 is adherent cell and detached by using trypsin-EDTA. however, over-digestion using trypsin might provoke cell clumping. they seemed like...
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When undertaking clinical trials with limited sample size and repeated measures, the question arises: which is more advantageous—employing "Generalized Estimating Equations (GEE)" or opting for...
21 January 2024 1,357 3 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...
06 June 2024 7,764 1 View
It can be found in most literatures and reviews that METTL3 exerts its methylase activity in the nucleus, interacting with adaptors such as METTL14 and WTAP, but is it possible for METTL3 to play...
09 April 2024 9,801 0 View
Hello everyone We are building an atomic structure model against a cryo-EM map of AAA+ ATPase. Considering that AAA+ polymerizes when ATP is added, we collected data for AAA+ mixed with ATP. Our...
04 April 2024 4,640 2 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
Hello! I'm plannning to investigate the relationship between mitochondrial mass/function and epithelial homeostasis/differentiation. Are there any activators(drug) of mitochondria interms of...
27 March 2024 8,273 0 View
What is the bubble in the cytoplasm as the figure I attached below? It also can be released into the culture medium after the cell dead.
18 March 2024 1,410 0 View
I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.
27 February 2024 6,940 1 View
We are right now researching a mitochondrial protein and intending to find the proteins it interacts with. Therefore, we did CoIP and MS/MS. Interestingly, several proteins are cytoplasmic...
31 January 2024 3,192 2 View
How to perform Western blot for GPI-anchored proteins, and are there any differences in the experimental conditions, particularly in the extraction and processing procedures of protein samples,...
28 January 2024 4,280 0 View