Geldanamycin is a well known Hsp90 Inhibitor (N-terminal). Does anyone have any idea how stable it is at higher temperatures (say 45-50 degrees) and pH above 7. I have to carry out some aggregation studies for 30-40 mins. I would use buffer in which protein is kept. so it could be around 7.4-7.8. I am not getting any reference in the web. Kindly get back to me if you have some relevant data.
Geldanamycin is soluble (5 mg/ml) in dichloromethane,
yielding a clear yellow-orange solution. It is also soluble
in DMSO (10 mg/ml). Insoluble in water.
Directions for Use: Geldanamycin is supplied as 600 μg
powder. Store at or below -20°C. Before usage, dissolve the
chemical in 1.08 ml DMSO to make 1 mM Geldanamycin.
For working concentrations of 300 nM-1 μM, dilute 1:3000
to 1:1000. Treat cells with the desired concentration for 20-
48 hours. Store liquid form at or below -20°C.
Note: Geldanamycin is not stable in liquid form (quality
decreased in 1-2 weeks). Use freshly dissolved geldanamycin
each time.