What is the easy way to make 6x loading dye?

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Malcolm Nobre

Hello B R Siddharth

You may use the protocol provided in the link below.

https://www.cytographica.com/lab/solutions/loadingdye.htm

Glycerol increases the density of the sample, encouraging it to sink when dispensed into wells, while the dyes provide a convenient marker for evaluating the progress of the run. For a given gel concentration, xylene cyanol FF runs toward the middle of the range of appropriate DNA lengths, while bromophenol blue runs toward the shorter end of the range.

Best.

Sandali Geethma

Hellow B R Siddharth

I am using 0.25% (w/v) Bromophenol blue, 0.25% (w/v) xylene cyanol FF, and 30% (w/v) glycerol in H20. Preparing according to "Molecular Cloning: A laboratory manual" by Joseph Sambrook. Refer to the appendices in this book.

Moslem Ghasemi

We used this method and got good results. My suggestion is to use this website.

https://www.laboratorynotes.com/preparation-of-6x-dna-loading-dye-bromophenol-blue-xylene-cyanol-ff-sucrose/#:~:text=Step%201%3A%20To%20prepare%2010,until%20all%20ingredients%20dissolve%20completely.