reverse genetics platform is used for cloning platform where cDNA is made from Viral RNA and is used for cloning
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...
22 February 2021 4,352 2 View
can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...
07 February 2021 470 2 View
Dear Researchers, In Textile Reinforced Concrete is a novel construction material consisting of a cementitious binder and a textile reinforcement. Without using a course aggregate why the topic...
24 January 2021 2,654 9 View
Shouldn't every individual who takes COVID-19 vaccination undergo periodic review to the study the state of corresponding antibodies? Individuals who do not produce any symptoms of COVID-19 after...
23 January 2021 6,170 2 View
Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...
21 January 2021 5,546 32 View
Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...
11 January 2021 9,500 2 View
Pesticides become prime compo in agricultural activities to meet the growing demand of the human population. But indiscriminate usage and mishandling led to two disasters in India such as Bhopal...
11 January 2021 3,790 7 View
I have a xrd pattern, in that i suspect that one of the peak may be due to SL, i want to understand how to confirm it or reject it
07 January 2021 5,534 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...
25 February 2021 2,388 3 View