transformation after ligation is nessesary step to get desired product in large amount.
I'm not sure precisely what question you are asking. But if it why does ligated produced transform less than a control plasmid, the explanation is that with a control plasmid 100% of the molecules are transformable. Whereas with a ligation product there are many many products from the ligation reaction that are not transformable, non circular, etc.
dear B R Siddharth
I think you mean why the rate of successful bacterial transformation is different from the ligation product and normal plasmid, (and the ligation product transformation rate is much less).
The reason is that the number of plasmids that perform successful ligation is low, and in addition, the ligation product is much more sensitive to endonucleases (because of DNA nick ), so the result of ligation process is a much smaller amount of healthy and replicable plasmid is available to the bacteria. Then the transformation result is a smaller number of colonies compared to transformation with a pure and healthy plasmid.
If you are asking why you need to perform the transformation after ligation to get lots of vector it’s because the bacteria replicate the vector.
if you are wondering why you get a lower transformation efficiently with ligation reactions than pure pre-made plasmid, Michael j benedik has a great answer
I don't see a valid reason for which there would be less bacteria transformed by the recombinant plasmid, just maybe the quantity of recombinant plasmid was diluted by the addition of the solution of the insert, the ligase and its buffer , which gives a result of a less good transformation of bacteria in comparison with that obtained with the non-recombined plasmid which is more concentrated. Add to that the possibility that the ligation was not very effective.
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