Azimun Nahar

10 Questions 32 Answers 0 Followers

Questions related from Azimun Nahar

How can I store in scientific way whole lettuce leaves after inoculation of E. coli O104 to see the survivability of that strain of E. coli?

I inoculated E.coli O104 in 6 whole lettuce (iceberg) leaves to see the survibality. I want to do 3 days interval sampling. Now I cannot understand whether I can keep individual leave in separate...

08 July 2015 4,878 3 View

Why does more count of cfu/ml of E.coli O104 come on LB with antibiotic than only LB post inoculation in Lettuce?

Dear Researcher I inoculated E.coli O104 in Lettuce and I did cfu counting after spreading on LB with antibiotic ( resistant of E.coli O104 and used to prevent normal flora growth) and on LB...

08 April 2015 4,184 9 View

How I can differentiate Enteropathogenic E. coli and Enterohemorrhagic E. coli at the molecular level?

I collected diarrhoeal calf feces and I detected the genes, eae, stx1 and 2, elt,est. A I found 2 eae positive strains which are negative for stx's and elt and est. Now can I say my 2 eae...

20 January 2015 8,576 4 View

Why does the presence of antibiotic like cefotaxime in agar media cannot prevent the growth of background flora in Lettuce?

I inoculated E.coli O104 in lettuce and did cfu counting after spreading in LB plate containing cefotaxime as well as MacConkey containing cefotaxime parallaly but in both case I found lot of of...

20 January 2015 8,118 9 View

Is there any effect of visual light exposure on E.coli growth?

I inoculated E. coli O104 in orange juice in a flask and kept it in the fridge in front of which there is light that lab personnel commonly use while working in lab at least 12 hours. Now I am...

05 January 2015 3,580 10 View

Does the speed of the vortex depend on the amount and viscosity the bacterial culture?

whether the speed and time of vortex depends on amount and viscosity of sample ( bacterial culture) or not. If depend, then for 5000 and 500 microliter of sample ( bacterial culture in juice )how...

24 December 2014 9,755 4 View

How do I homogenize a bacterial sample with a vortex before spreading?

For spreading of 100 microlitre of bacterial culture in juice, how long and what method I have to follow for vortex of 500 micolitre of bacterial culture?

23 December 2014 5,726 6 View

How can I show a standard error with the mean of triplicate plate count in MS Excel?

I did triplicate plate count for cfu counting of my bacteria on LB agar plate at 3 days interval until 21 days. Now I want to show every sampling, mean of 3 plate counting and their standard error...

22 December 2014 5,634 2 View

Which factors can affect the survivability of E.coli in orange juice?

I inoculated E.coli C600 in orange juice but it can survive until 6-9 days. Why?

20 December 2014 1,911 16 View

How can I prevent fungal growth in orange juice after inoculating E. coli O104 to see the BVNC state?

I want to see the VBNC state of E. coli O104 and O157 in orange juice. I inoculated those bacteria in orange juice but after certain time I found so much fungal growth in inoculated range juice....

16 December 2014 7,290 13 View