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Questions related from Azimun Nahar
I did PFGE with S-1 nuclease digestion of some ESBL-producing E. coli isolates. Some isolates showed good bands but some isolates showed very faint bands after gel electrophoresis. So, how to...
21 December 2017 6,921 1 View
I inoculated E.coli O104 in 6 whole lettuce (iceberg) leaves to see the survibality. I want to do 3 days interval sampling. Now I cannot understand whether I can keep individual leave in separate...
08 July 2015 4,895 3 View
I want to see the survivability of E. coli O104 in whole lettuce leaves. So I want to know how exactly I can inoculate of above mentioned bacteria in lettuce leaves. If anybody know the procedure...
26 May 2015 6,843 11 View
Dear Researcher I inoculated E.coli O104 in Lettuce and I did cfu counting after spreading on LB with antibiotic ( resistant of E.coli O104 and used to prevent normal flora growth) and on LB...
08 April 2015 4,194 9 View
I tried to grow E. coli C600 in LB broth from a glycerol stock kept in -80 degree celsius and I used fresh LB plate and broth for it and tried 3 times to grow it but every time it grew very slowly...
05 February 2015 8,386 21 View
I collected diarrhoeal calf feces and I detected the genes, eae, stx1 and 2, elt,est. A I found 2 eae positive strains which are negative for stx's and elt and est. Now can I say my 2 eae...
20 January 2015 8,587 4 View
I inoculated E.coli O104 in lettuce and did cfu counting after spreading in LB plate containing cefotaxime as well as MacConkey containing cefotaxime parallaly but in both case I found lot of of...
20 January 2015 8,129 9 View
I inoculated E. coli O104 in orange juice in a flask and kept it in the fridge in front of which there is light that lab personnel commonly use while working in lab at least 12 hours. Now I am...
05 January 2015 3,595 10 View
When I spray alcohol on safety cabinet or rub instruments with alcohol to sterilize it, then I want to start my experiment in safety cabinet with that sterilized instruments. Now I wan to know...
28 December 2014 542 4 View
whether the speed and time of vortex depends on amount and viscosity of sample ( bacterial culture) or not. If depend, then for 5000 and 500 microliter of sample ( bacterial culture in juice )how...
24 December 2014 9,763 4 View
For spreading of 100 microlitre of bacterial culture in juice, how long and what method I have to follow for vortex of 500 micolitre of bacterial culture?
23 December 2014 5,734 6 View
I did triplicate plate count for cfu counting of my bacteria on LB agar plate at 3 days interval until 21 days. Now I want to show every sampling, mean of 3 plate counting and their standard error...
22 December 2014 5,647 2 View
I inoculated E.coli C600 in orange juice but it can survive until 6-9 days. Why?
20 December 2014 1,921 16 View
I want to see the VBNC state of E. coli O104 and O157 in orange juice. I inoculated those bacteria in orange juice but after certain time I found so much fungal growth in inoculated range juice....
16 December 2014 7,300 13 View
