The World Organization for Animal Health (OIE) recommends a minimum and maximum range of reagent concentrations to be followed by laboratories performing the complement fixation (CFT) for livestock brucellosis in order to maintain interlab harmony of results. Different labs worldwide use different CFT versions that either cope completely or mostly with OIE recommendations leading to some variations in test sensitivity that suit the epidemiological situation of each country. For instance, the modified Australian method published in Alton et al. (1988) uses 2 full units of antigen, 5 half units of complement, 3% RBCs and 1.25 full unit of hemolysin. The French CFT, on the other hand, uses 1 half unit of antigen, 6 half units of complement, 2.5% RBCs and 2 full units of hemolysin.

On what technical grounds does every country choose the combination of concentrations/ hemolytic units of complement fixation test reagents for the titration and hence conduct the test properly?

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