Cell lines to evaluate cytotoxicity of drug?

Hi Everyone, I am working on some drugs for HSV. I am wondering what are the best heart, liver and kidney cells lines to evaluate cytotoxicity of my drug on heart, liver and kidney...

17 June 2019 7,591 2 View

Hi , I wanted to collect exosomes from cell culture media through ultracentrifugation.but i couldn't see pallet. Why?

I collected media in two bottles and centrifuge for 800g RCF for 10 minutes . i couldn't see any pallet , then i went for 2000g for 20 minutes still nothing what should be the possible reason?

27 December 2018 5,642 3 View

I want to collect blood of normal mouse after treating it with Ag to check drug uptake, What is best way to collect blood?

doing animal model, for cancer related research

19 December 2018 2,428 3 View

My pure anabaen sample is contaminated with chlorella vulgaris. How to get rid of chlorella and keep anabaen pure?

I am facing problem regarding purification of anabaena. i have used serial dilution and plate streaking method. But everytime the culture is contaminated with chlorella vulgaris. I have to perform...

12 November 2018 7,869 3 View

I fabricated GNP by reduction of sodium citrate but its colour is not bright red ,, what might be the reason ?

Fabrication of GNP

28 May 2018 9,702 1 View

How wind speed is mathematically related wind power???

How wind speed is mathematically related wind power???

23 February 2018 1,959 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View